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Items: 1 to 20 of 115

1.

Single-mRNA counting using fluorescent in situ hybridization in budding yeast.

Trcek T, Chao JA, Larson DR, Park HY, Zenklusen D, Shenoy SM, Singer RH.

Nat Protoc. 2012 Feb 2;7(2):408-19. doi: 10.1038/nprot.2011.451.

2.

Visualization and analysis of mRNA molecules using fluorescence in situ hybridization in Saccharomyces cerevisiae.

McIsaac RS, Silverman SJ, Parsons L, Xu P, Briehof R, McClean MN, Botstein D.

J Vis Exp. 2013 Jun 14;(76):e50382. doi: 10.3791/50382.

3.

mRNA quantification using single-molecule FISH in Drosophila embryos.

Trcek T, Lionnet T, Shroff H, Lehmann R.

Nat Protoc. 2017 Jul;12(7):1326-1348. doi: 10.1038/nprot.2017.030. Epub 2016 Jun 8.

PMID:
28594816
4.

Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae.

Rahman S, Zenklusen D.

Methods Mol Biol. 2013;1042:33-46. doi: 10.1007/978-1-62703-526-2_3.

PMID:
23979998
5.

Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization.

Skinner SO, Sepúlveda LA, Xu H, Golding I.

Nat Protoc. 2013 Jun;8(6):1100-13. doi: 10.1038/nprot.2013.066. Epub 2013 May 16. Erratum in: Nat Protoc. 2015 Sep;10(9):1457.

6.
7.

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast.

Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH.

Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23.

8.

Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization.

Zenklusen D, Singer RH.

Methods Enzymol. 2010;470:641-59. doi: 10.1016/S0076-6879(10)70026-4. Epub 2010 Mar 1.

9.

Evaluating gene expression dynamics using pairwise RNA FISH data.

Wyart M, Botstein D, Wingreen NS.

PLoS Comput Biol. 2010 Nov 4;6(11):e1000979. doi: 10.1371/journal.pcbi.1000979.

10.

Monitoring the temporal and spatial distribution of RNA in living yeast cells.

Long RM, Urbinati CR.

Methods Mol Biol. 2008;419:187-96. doi: 10.1007/978-1-59745-033-1_13.

PMID:
18369984
12.
13.

Simultaneous detection of nuclear and cytoplasmic RNA variants utilizing Stellaris® RNA fluorescence in situ hybridization in adherent cells.

Coassin SR, Orjalo AV Jr, Semaan SJ, Johansson HE.

Methods Mol Biol. 2014;1211:189-99. doi: 10.1007/978-1-4939-1459-3_15.

PMID:
25218386
14.

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells.

Orjalo AV Jr, Johansson HE.

Methods Mol Biol. 2016;1402:119-134. doi: 10.1007/978-1-4939-3378-5_10.

PMID:
26721487
15.

RNA detection in situ with FISH-STICs.

Sinnamon JR, Czaplinski K.

RNA. 2014 Feb;20(2):260-6. doi: 10.1261/rna.041905.113. Epub 2013 Dec 17.

16.

Dramatically improved RNA in situ hybridization signals using LNA-modified probes.

Thomsen R, Nielsen PS, Jensen TH.

RNA. 2005 Nov;11(11):1745-8. Epub 2005 Sep 21.

17.

Quantitative gene expression analysis in Caenorhabditis elegans using single molecule RNA FISH.

Bolková J, Lanctôt C.

Methods. 2016 Apr 1;98:42-49. doi: 10.1016/j.ymeth.2015.11.008. Epub 2015 Nov 10.

PMID:
26564238
18.

Single-molecule mRNA detection and counting in mammalian tissue.

Lyubimova A, Itzkovitz S, Junker JP, Fan ZP, Wu X, van Oudenaarden A.

Nat Protoc. 2013 Sep;8(9):1743-58. doi: 10.1038/nprot.2013.109. Epub 2013 Aug 15.

PMID:
23949380
19.

Visualizing mRNAs in fixed and living yeast cells.

Gallardo F, Chartrand P.

Methods Mol Biol. 2011;714:203-19. doi: 10.1007/978-1-61779-005-8_13.

PMID:
21431743
20.

Segmentation and detection of fluorescent 3D spots.

Ram S, Rodríguez JJ, Bosco G.

Cytometry A. 2012 Mar;81(3):198-212. doi: 10.1002/cyto.a.22017.

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