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Items: 1 to 20 of 102

1.

A pipeline for the generation of shRNA transgenic mice.

Dow LE, Premsrirut PK, Zuber J, Fellmann C, McJunkin K, Miething C, Park Y, Dickins RA, Hannon GJ, Lowe SW.

Nat Protoc. 2012 Feb 2;7(2):374-93. doi: 10.1038/nprot.2011.446.

2.

A rapid and scalable system for studying gene function in mice using conditional RNA interference.

Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, Hannon GJ, Lowe SW.

Cell. 2011 Apr 1;145(1):145-58. doi: 10.1016/j.cell.2011.03.012.

3.

Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cells.

Cheng TL, Chang WT.

Methods Mol Biol. 2007;408:223-41. doi: 10.1007/978-1-59745-547-3_13.

PMID:
18314586
4.

Target validation in mice by constitutive and conditional RNAi.

Kleinhammer A, Wurst W, Kühn R.

Methods Mol Biol. 2013;986:307-23. doi: 10.1007/978-1-62703-311-4_19.

PMID:
23436420
5.

Conditional RNAi in mice.

Kleinhammer A, Deussing J, Wurst W, Kühn R.

Methods. 2011 Feb;53(2):142-50. doi: 10.1016/j.ymeth.2010.08.003. Epub 2010 Aug 10. Review.

PMID:
20705138
6.

Constitutive and conditional RNAi transgenesis in mice.

Kleinhammer A, Wurst W, Kühn R.

Methods. 2011 Apr;53(4):430-6. doi: 10.1016/j.ymeth.2010.12.015. Epub 2010 Dec 22.

PMID:
21184828
7.

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.

Sarnova L, Malik R, Sedlacek R, Svoboda P.

J Negat Results Biomed. 2010 Oct 12;9:8. doi: 10.1186/1477-5751-9-8.

8.

A simple and robust vector-based shRNA expression system used for RNA interference.

Wang XJ, Li Y, Huang H, Zhang XJ, Xie PW, Hu W, Li DD, Wang SQ.

PLoS One. 2013;8(2):e56110. doi: 10.1371/journal.pone.0056110. Epub 2013 Feb 6.

9.

Mammalian RNAi: a practical guide.

Sandy P, Ventura A, Jacks T.

Biotechniques. 2005 Aug;39(2):215-24. Review.

10.

A resource for large-scale RNA-interference-based screens in mammals.

Paddison PJ, Silva JM, Conklin DS, Schlabach M, Li M, Aruleba S, Balija V, O'Shaughnessy A, Gnoj L, Scobie K, Chang K, Westbrook T, Cleary M, Sachidanandam R, McCombie WR, Elledge SJ, Hannon GJ.

Nature. 2004 Mar 25;428(6981):427-31.

PMID:
15042091
11.

Generation of shRNA transgenic mice.

Hitz C, Steuber-Buchberger P, Delic S, Wurst W, Kühn R.

Methods Mol Biol. 2009;530:101-29. doi: 10.1007/978-1-59745-471-1_6.

PMID:
19266327
12.

RNA interference in mice.

Kühn R, Streif S, Wurst W.

Handb Exp Pharmacol. 2007;(178):149-76. Review.

PMID:
17203655
13.

Gene manipulation through the use of small interfering RNA (siRNA): from in vitro to in vivo applications.

Kumar LD, Clarke AR.

Adv Drug Deliv Rev. 2007 Mar 30;59(2-3):87-100. Epub 2007 Mar 20. Review.

PMID:
17434644
14.

A quick and efficient approach for gene silencing by using triple putative microRNA-based short hairpin RNAs.

Shan ZX, Lin QX, Yang M, Deng CY, Kuang SJ, Zhou ZL, Xiao DZ, Liu XY, Lin SG, Yu XY.

Mol Cell Biochem. 2009 Mar;323(1-2):81-9. doi: 10.1007/s11010-008-9966-3. Epub 2008 Nov 27.

PMID:
19037714
15.

Design of lentivirally expressed siRNAs.

Liu YP, Berkhout B.

Methods Mol Biol. 2013;942:233-57. doi: 10.1007/978-1-62703-119-6_13.

PMID:
23027055
16.

A genome-scale shRNA resource for transgenic RNAi in Drosophila.

Ni JQ, Zhou R, Czech B, Liu LP, Holderbaum L, Yang-Zhou D, Shim HS, Tao R, Handler D, Karpowicz P, Binari R, Booker M, Brennecke J, Perkins LA, Hannon GJ, Perrimon N.

Nat Methods. 2011 May;8(5):405-7. doi: 10.1038/nmeth.1592. Epub 2011 Apr 3.

17.

Lentiviral miR30-based RNA interference against heparanase suppresses melanoma metastasis with lower liver and lung toxicity.

Liu XY, Tang QS, Chen HC, Jiang XL, Fang H.

Int J Biol Sci. 2013 Jun 12;9(6):564-77. doi: 10.7150/ijbs.5425. Print 2013.

18.

A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector.

Frank SB, Schulz VV, Miranti CK.

BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x.

19.

Organic small hairpin RNAs (OshR): a do-it-yourself platform for transgene-based gene silencing.

Zeng M, Kuzirian MS, Harper L, Paradis S, Nakayama T, Lau NC.

Methods. 2013 Sep 15;63(2):101-9. doi: 10.1016/j.ymeth.2013.05.007. Epub 2013 May 23.

20.

Functional identification of optimized RNAi triggers using a massively parallel sensor assay.

Fellmann C, Zuber J, McJunkin K, Chang K, Malone CD, Dickins RA, Xu Q, Hengartner MO, Elledge SJ, Hannon GJ, Lowe SW.

Mol Cell. 2011 Mar 18;41(6):733-46. doi: 10.1016/j.molcel.2011.02.008. Epub 2011 Feb 25.

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