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Items: 1 to 20 of 100

1.

High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.

Bird AW, Erler A, Fu J, Hériché JK, Maresca M, Zhang Y, Hyman AA, Stewart AF.

Nat Methods. 2011 Dec 4;9(1):103-9. doi: 10.1038/nmeth.1803.

PMID:
22138824
2.

Recombineering-based procedure for creating BAC transgene constructs for animals and cell lines.

Hollenback SM, Lyman S, Cheng J.

Curr Protoc Mol Biol. 2011 Jul;Chapter 23:Unit 23.14. doi: 10.1002/0471142727.mb2314s95.

3.

Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis.

Lyozin GT, Bressloff PC, Kumar A, Kosaka Y, Demarest BL, Yost HJ, Kuehn MR, Brunelli L.

Nat Methods. 2014 Sep;11(9):966-970. doi: 10.1038/nmeth.3030. Epub 2014 Jul 13.

4.

Improved seamless mutagenesis by recombineering using ccdB for counterselection.

Wang H, Bian X, Xia L, Ding X, Müller R, Zhang Y, Fu J, Stewart AF.

Nucleic Acids Res. 2014 Mar;42(5):e37. doi: 10.1093/nar/gkt1339. Epub 2013 Dec 24.

5.

A new positive/negative selection scheme for precise BAC recombineering.

Wang S, Zhao Y, Leiby M, Zhu J.

Mol Biotechnol. 2009 May;42(1):110-6. doi: 10.1007/s12033-009-9142-3. Epub 2009 Jan 22.

6.

Insertion of modifications in the beta-globin locus using GET recombination with single-stranded oligonucleotides and denatured PCR fragments.

Jamsai D, Orford M, Fucharoen S, Williamson R, Ioannou PA.

Mol Biotechnol. 2003 Jan;23(1):29-36.

PMID:
12611267
7.

Point mutation of bacterial artificial chromosomes by ET recombination.

Muyrers JP, Zhang Y, Benes V, Testa G, Ansorge W, Stewart AF.

EMBO Rep. 2000 Sep;1(3):239-43.

9.

Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA.

Hager S, Lösch S, Noll S, Khan-Vaughan L, Ehrlich ME, Kranz H.

Biotechniques. 2012 Jul 1;53(1). doi: 10.2144/000113908.

10.

General method for the modification of different BAC types and the rapid generation of BAC transgenic mice.

Sparwasser T, Gong S, Li JY, Eberl G.

Genesis. 2004 Jan;38(1):39-50.

PMID:
14755803
11.

Modification of bacterial artificial chromosomes (BACs) and preparation of intact BAC DNA for generation of transgenic mice.

Gong S, Yang XW.

Curr Protoc Neurosci. 2005 May;Chapter 5:Unit 5.21. doi: 10.1002/0471142301.ns0521s31.

PMID:
18428623
12.

Rapid BAC selection for tol2-mediated transgenesis in zebrafish.

Bussmann J, Schulte-Merker S.

Development. 2011 Oct;138(19):4327-32. doi: 10.1242/dev.068080. Epub 2011 Aug 24.

13.

An overview on the generation of BAC transgenic mice for neuroscience research.

Yang XW, Gong S.

Curr Protoc Neurosci. 2005 May;Chapter 5:Unit 5.20. doi: 10.1002/0471142301.ns0520s31. Review.

PMID:
18428622
14.

Insertion of common mutations into the human beta-globin locus using GET Recombination and an EcoRI endonuclease counterselection cassette.

Jamsai D, Nefedov M, Narayanan K, Orford M, Fucharoen S, Williamson R, Ioannou PA.

J Biotechnol. 2003 Feb 27;101(1):1-9.

PMID:
12523964
15.

Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets.

van Kessel JC, Hatfull GF.

Mol Microbiol. 2008 Mar;67(5):1094-107. doi: 10.1111/j.1365-2958.2008.06109.x. Epub 2008 Jan 22.

17.

Modification and production of BAC transgenes.

Jeong Y, Epstein DJ.

Curr Protoc Mol Biol. 2005 Aug;Chapter 23:Unit 23.11. doi: 10.1002/0471142727.mb2311s71.

PMID:
18265362
18.

Efficient recombination-based methods for bacterial artificial chromosome fusion and mutagenesis.

Sopher BL, La Spada AR.

Gene. 2006 Apr 12;371(1):136-43. Epub 2006 Feb 17.

PMID:
16487669
19.

REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering.

Trehan A, Kiełbus M, Czapinski J, Stepulak A, Huhtaniemi I, Rivero-Müller A.

Sci Rep. 2016 Jan 11;6:19121. doi: 10.1038/srep19121.

20.

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.

Poser I, Sarov M, Hutchins JR, Hériché JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA.

Nat Methods. 2008 May;5(5):409-15. doi: 10.1038/nmeth.1199. Epub 2008 Apr 6. Erratum in: Nat Methods. 2008 Aug;5(8):748.

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