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Items: 1 to 20 of 132

1.

PHUSER (Primer Help for USER): a novel tool for USER fusion primer design.

Olsen LR, Hansen NB, Bonde MT, Genee HJ, Holm DK, Carlsen S, Hansen BG, Patil KR, Mortensen UH, Wernersson R.

Nucleic Acids Res. 2011 Jul;39(Web Server issue):W61-7. doi: 10.1093/nar/gkr394.

2.

USER-derived cloning methods and their primer design.

Salomonsen B, Mortensen UH, Halkier BA.

Methods Mol Biol. 2014;1116:59-72. doi: 10.1007/978-1-62703-764-8_5.

PMID:
24395357
3.

Software-supported USER cloning strategies for site-directed mutagenesis and DNA assembly.

Genee HJ, Bonde MT, Bagger FO, Jespersen JB, Sommer MO, Wernersson R, Olsen LR.

ACS Synth Biol. 2015 Mar 20;4(3):342-9. doi: 10.1021/sb500194z.

PMID:
24847672
4.

RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis-priming from structural variation.

Piriyapongsa J, Ngamphiw C, Assawamakin A, Wangkumhang P, Suwannasri P, Ruangrit U, Agavatpanitch G, Tongsima S.

BMC Genomics. 2009 Dec 3;10 Suppl 3:S4. doi: 10.1186/1471-2164-10-S3-S4.

5.

MFEprimer: multiple factor evaluation of the specificity of PCR primers.

Qu W, Shen Z, Zhao D, Yang Y, Zhang C.

Bioinformatics. 2009 Jan 15;25(2):276-8. doi: 10.1093/bioinformatics/btn614.

6.

Primer Design Assistant (PDA): A web-based primer design tool.

Chen SH, Lin CY, Cho CS, Lo CZ, Hsiung CA.

Nucleic Acids Res. 2003 Jul 1;31(13):3751-4.

7.

QuantPrime--a flexible tool for reliable high-throughput primer design for quantitative PCR.

Arvidsson S, Kwasniewski M, Riaño-Pachón DM, Mueller-Roeber B.

BMC Bioinformatics. 2008 Nov 1;9:465. doi: 10.1186/1471-2105-9-465.

8.

Graphical design of primers with PerlPrimer.

Marshall O.

Methods Mol Biol. 2007;402:403-14. Review.

PMID:
17951808
9.

Amplicon: software for designing PCR primers on aligned DNA sequences.

Jarman SN.

Bioinformatics. 2004 Jul 10;20(10):1644-5.

10.

DePIE: Designing Primers for Protein Interaction Experiments.

Lu G, Hallett M, Pollock S, Thomas D.

Nucleic Acids Res. 2003 Jul 1;31(13):3755-7.

11.

WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations.

Wangkumhang P, Chaichoompu K, Ngamphiw C, Ruangrit U, Chanprasert J, Assawamakin A, Tongsima S.

BMC Genomics. 2007 Aug 14;8:275.

12.
13.

Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL.

BMC Bioinformatics. 2012 Jun 18;13:134. doi: 10.1186/1471-2105-13-134.

14.

Genome-scale probe and primer design with PRIMEGENS.

Srivastava GP, Xu D.

Methods Mol Biol. 2007;402:159-76. Review.

PMID:
17951795
15.

Oligonucleotide properties determination and primer designing: a critical examination of predictions.

Chavali S, Mahajan A, Tabassum R, Maiti S, Bharadwaj D.

Bioinformatics. 2005 Oct 15;21(20):3918-25. Erratum in: Bioinformatics. 2005 Nov 15;21(22):4198.

16.

RF-Cloning.org: an online tool for the design of restriction-free cloning projects.

Bond SR, Naus CC.

Nucleic Acids Res. 2012 Jul;40(Web Server issue):W209-13. doi: 10.1093/nar/gks396.

17.

Design of oligonucleotides for microarrays and perspectives for design of multi-transcriptome arrays.

Nielsen HB, Wernersson R, Knudsen S.

Nucleic Acids Res. 2003 Jul 1;31(13):3491-6.

18.

Assembling DNA fragments by USER fusion.

Annaluru N, Muller H, Ramalingam S, Kandavelou K, London V, Richardson SM, Dymond JS, Cooper EM, Bader JS, Boeke JD, Chandrasegaran S.

Methods Mol Biol. 2012;852:77-95. doi: 10.1007/978-1-61779-564-0_7.

PMID:
22328427
19.

Primer3Plus, an enhanced web interface to Primer3.

Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JA.

Nucleic Acids Res. 2007 Jul;35(Web Server issue):W71-4.

20.

AutoDimer: a screening tool for primer-dimer and hairpin structures.

Vallone PM, Butler JM.

Biotechniques. 2004 Aug;37(2):226-31.

PMID:
15335214
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