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Items: 1 to 20 of 100

1.

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study.

Busso D, Peleg Y, Heidebrecht T, Romier C, Jacobovitch Y, Dantes A, Salim L, Troesch E, Schuetz A, Heinemann U, Folkers GE, Geerlof A, Wilmanns M, Polewacz A, Quedenau C, Büssow K, Adamson R, Blagova E, Walton J, Cartwright JL, Bird LE, Owens RJ, Berrow NS, Wilson KS, Sussman JL, Perrakis A, Celie PH.

J Struct Biol. 2011 Aug;175(2):159-70. doi: 10.1016/j.jsb.2011.03.004. Epub 2011 Mar 5.

PMID:
21382497
2.

Deciphering correct strategies for multiprotein complex assembly by co-expression: application to complexes as large as the histone octamer.

Diebold ML, Fribourg S, Koch M, Metzger T, Romier C.

J Struct Biol. 2011 Aug;175(2):178-88. doi: 10.1016/j.jsb.2011.02.001. Epub 2011 Feb 12.

PMID:
21320604
3.

Characterization and production of protein complexes by co-expression in Escherichia coli.

Haffke M, Marek M, Pelosse M, Diebold ML, Schlattner U, Berger I, Romier C.

Methods Mol Biol. 2015;1261:63-89. doi: 10.1007/978-1-4939-2230-7_4.

PMID:
25502194
4.

Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells.

Vijayachandran LS, Viola C, Garzoni F, Trowitzsch S, Bieniossek C, Chaillet M, Schaffitzel C, Busso D, Romier C, Poterszman A, Richmond TJ, Berger I.

J Struct Biol. 2011 Aug;175(2):198-208. doi: 10.1016/j.jsb.2011.03.007. Epub 2011 Mar 17. Review.

PMID:
21419851
5.

Strategies for the structural analysis of multi-protein complexes: lessons from the 3D-Repertoire project.

Collinet B, Friberg A, Brooks MA, van den Elzen T, Henriot V, Dziembowski A, Graille M, Durand D, Leulliot N, Saint André C, Lazar N, Sattler M, Séraphin B, van Tilbeurgh H.

J Struct Biol. 2011 Aug;175(2):147-58. doi: 10.1016/j.jsb.2011.03.018. Epub 2011 Apr 2.

PMID:
21463689
6.

Gene selection and cloning approaches for co-expression and production of recombinant protein-protein complexes.

Babnigg G, Jedrzejczak R, Nocek B, Stein A, Eschenfeldt W, Stols L, Marshall N, Weger A, Wu R, Donnelly M, Joachimiak A.

J Struct Funct Genomics. 2015 Dec;16(3-4):113-28. doi: 10.1007/s10969-015-9200-y. Epub 2015 Dec 15.

PMID:
26671275
7.

Resolving bottlenecks for recombinant protein expression in E. coli.

Peleg Y, Unger T.

Methods Mol Biol. 2012;800:173-86. doi: 10.1007/978-1-61779-349-3_12.

PMID:
21964789
8.

Strategies for the production of recombinant protein in Escherichia coli.

Gopal GJ, Kumar A.

Protein J. 2013 Aug;32(6):419-25. doi: 10.1007/s10930-013-9502-5. Review.

PMID:
23897421
9.

Recombinant protein expression and solubility screening in Escherichia coli: a comparative study.

Berrow NS, Büssow K, Coutard B, Diprose J, Ekberg M, Folkers GE, Levy N, Lieu V, Owens RJ, Peleg Y, Pinaglia C, Quevillon-Cheruel S, Salim L, Scheich C, Vincentelli R, Busso D.

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1218-26. Epub 2006 Sep 19.

PMID:
17001098
10.

Medium-throughput production of recombinant human proteins: ligation-independent cloning.

Strain-Damerell C, Mahajan P, Gileadi O, Burgess-Brown NA.

Methods Mol Biol. 2014;1091:55-72. doi: 10.1007/978-1-62703-691-7_4.

PMID:
24203324
11.

Application of high-throughput methodologies to the expression of recombinant proteins in E. coli.

Peleg Y, Unger T.

Methods Mol Biol. 2008;426:197-208. doi: 10.1007/978-1-60327-058-8_12.

PMID:
18542865
12.

Complex Reconstitution and Characterization by Combining Co-expression Techniques in Escherichia coli with High-Throughput.

Vincentelli R, Romier C.

Adv Exp Med Biol. 2016;896:43-58. doi: 10.1007/978-3-319-27216-0_4. Review.

PMID:
27165318
13.
14.

Technologies to keep an eye on: alternative hosts for protein production in structural biology.

Fernández FJ, Vega MC.

Curr Opin Struct Biol. 2013 Jun;23(3):365-73. doi: 10.1016/j.sbi.2013.02.002. Epub 2013 Mar 5. Review.

PMID:
23481468
15.

Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes.

Zeghouf M, Li J, Butland G, Borkowska A, Canadien V, Richards D, Beattie B, Emili A, Greenblatt JF.

J Proteome Res. 2004 May-Jun;3(3):463-8.

PMID:
15253427
16.

Construction of a compatible Gateway-based co-expression vector set for expressing multiprotein complexes in E. coli.

Salim L, Feger C, Busso D.

Anal Biochem. 2016 Nov 1;512:110-113. doi: 10.1016/j.ab.2016.08.018. Epub 2016 Aug 21.

PMID:
27558914
17.
18.

Engineering of a wheat germ expression system to provide compatibility with a high throughput pET-based cloning platform.

Zhao L, Zhao KQ, Hurst R, Slater MR, Acton TB, Swapna GV, Shastry R, Kornhaber GJ, Montelione GT.

J Struct Funct Genomics. 2010 Sep;11(3):201-9. doi: 10.1007/s10969-010-9093-8. Epub 2010 Jun 24.

19.

Novel vectors for co-expression of two proteins in E. coli.

Kholod N, Mustelin T.

Biotechniques. 2001 Aug;31(2):322-3, 326-8.

PMID:
11515368
20.

Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli.

Fribourg S, Romier C, Werten S, Gangloff YG, Poterszman A, Moras D.

J Mol Biol. 2001 Feb 16;306(2):363-73.

PMID:
11237605

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