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Directed evolution of the transcription factor XylS for development of improved expression systems.

Vee Aune TE, Bakke I, Drabløs F, Lale R, Brautaset T, Valla S.

Microb Biotechnol. 2010 Jan;3(1):38-47. doi: 10.1111/j.1751-7915.2009.00126.x. Epub 2009 Jun 3.


Identification of critical amino-terminal regions of XylS. The positive regulator encoded by the TOL plasmid.

Michan C, Zhou L, Gallegos MT, Timmis KN, Ramos JL.

J Biol Chem. 1992 Nov 15;267(32):22897-901.


Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression.

Bakke I, Berg L, Aune TE, Brautaset T, Sletta H, Tøndervik A, Valla S.

Appl Environ Microbiol. 2009 Apr;75(7):2002-11. doi: 10.1128/AEM.02315-08. Epub 2009 Feb 5.


Functional domains of the TOL plasmid transcription factor XylS.

Kaldalu N, Toots U, de Lorenzo V, Ustav M.

J Bacteriol. 2000 Feb;182(4):1118-26.


XylS-Pm promoter interactions through two helix-turn-helix motifs: identifying XylS residues important for DNA binding and activation.

Domínguez-Cuevas P, Marín P, Marqués S, Ramos JL.

J Mol Biol. 2008 Jan 4;375(1):59-69. Epub 2007 Oct 23.


Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding.

Domínguez-Cuevas P, Marín P, Busby S, Ramos JL, Marqués S.

J Bacteriol. 2008 May;190(9):3118-28. doi: 10.1128/JB.01784-07. Epub 2008 Feb 22.


XylS activator and RNA polymerase binding sites at the Pm promoter overlap.

González-Pérez MM, Marqués S, Domínguez-Cuevas P, Ramos JL.

FEBS Lett. 2002 May 22;519(1-3):117-22.


Continuous control of the flow in biochemical pathways through 5' untranslated region sequence modifications in mRNA expressed from the broad-host-range promoter Pm.

Lale R, Berg L, Stüttgen F, Netzer R, Stafsnes M, Brautaset T, Vee Aune TE, Valla S.

Appl Environ Microbiol. 2011 Apr;77(8):2648-55. doi: 10.1128/AEM.02091-10. Epub 2011 Feb 18.


Differences in the mechanism of the allosteric l-rhamnose responses of the AraC/XylS family transcription activators RhaS and RhaR.

Kolin A, Balasubramaniam V, Skredenske JM, Wickstrum JR, Egan SM.

Mol Microbiol. 2008 Apr;68(2):448-61. doi: 10.1111/j.1365-2958.2008.06164.x.


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