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Items: 1 to 20 of 115

1.

Screening of genetic parameters for soluble protein expression in Escherichia coli.

Vernet E, Kotzsch A, Voldborg B, Sundström M.

Protein Expr Purif. 2011 May;77(1):104-11. doi: 10.1016/j.pep.2010.11.016. Epub 2010 Dec 2.

PMID:
21130169
2.

EspA is a novel fusion partner for expression of foreign proteins in Escherichia coli.

Cheng Y, Gu J, Wang HG, Yu S, Liu YQ, Ning YL, Zou QM, Yu XJ, Mao XH.

J Biotechnol. 2010 Nov;150(3):380-8. doi: 10.1016/j.jbiotec.2010.09.940. Epub 2010 Sep 18.

PMID:
20854850
3.

Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins.

Braud S, Moutiez M, Belin P, Abello N, Drevet P, Zinn-Justin S, Courçon M, Masson C, Dassa J, Charbonnier JB, Boulain JC, Ménez A, Genet R, Gondry M.

J Proteome Res. 2005 Nov-Dec;4(6):2137-47.

PMID:
16335960
4.

Advanced genetic strategies for recombinant protein expression in Escherichia coli.

Sørensen HP, Mortensen KK.

J Biotechnol. 2005 Jan 26;115(2):113-28. Review.

PMID:
15607230
5.

Application of high-throughput methodologies to the expression of recombinant proteins in E. coli.

Peleg Y, Unger T.

Methods Mol Biol. 2008;426:197-208. doi: 10.1007/978-1-60327-058-8_12.

PMID:
18542865
6.

Systematic high-yield production of human secreted proteins in Escherichia coli.

Dai X, Chen Q, Lian M, Zhou Y, Zhou M, Lu S, Chen Y, Luo J, Gu X, Jiang Y, Luo M, Zheng X.

Biochem Biophys Res Commun. 2005 Jul 1;332(2):593-601.

PMID:
15896719
7.

High-throughput protein expression screening and purification in Escherichia coli.

Vincentelli R, Cimino A, Geerlof A, Kubo A, Satou Y, Cambillau C.

Methods. 2011 Sep;55(1):65-72. doi: 10.1016/j.ymeth.2011.08.010. Epub 2011 Sep 8.

PMID:
21925268
8.

[Influence of the reductase deficient Escherichia coli on the solubility of recombinant proteins produced in it].

Xiong S, Zhang MY, Qian CW, Ran YC, Wang YF, Ren XR, Su KY, Yu ZY.

Sheng Wu Gong Cheng Xue Bao. 2003 Nov;19(6):686-91. Chinese.

PMID:
15971580
9.

Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: advantages of high-throughput screening.

Correa A, Oppezzo P.

Biotechnol J. 2011 Jun;6(6):715-30. doi: 10.1002/biot.201100025. Epub 2011 May 12. Review.

PMID:
21567962
10.

An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability.

Fan J, Heng J, Dai S, Shaw N, Zhou B, Huang B, He Z, Wang Y, Jiang T, Li X, Liu Z, Wang X, Zhang XC.

Protein Expr Purif. 2011 Jul;78(1):6-13. doi: 10.1016/j.pep.2011.02.010. Epub 2011 Feb 24.

PMID:
21354311
11.

Expression of proteins using the third domain of the Escherichia coli periplasmic-protein TolA as a fusion partner.

Anderluh G, Gökçe I, Lakey JH.

Protein Expr Purif. 2003 Mar;28(1):173-81.

PMID:
12651122
12.

Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli.

Tsunoda Y, Sakai N, Kikuchi K, Katoh S, Akagi K, Miura-Ohnuma J, Tashiro Y, Murata K, Shibuya N, Katoh E.

Protein Expr Purif. 2005 Aug;42(2):268-77.

PMID:
15914031
13.
14.

HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification.

Ohana RF, Encell LP, Zhao K, Simpson D, Slater MR, Urh M, Wood KV.

Protein Expr Purif. 2009 Nov;68(1):110-20. doi: 10.1016/j.pep.2009.05.010. Epub 2009 May 21.

PMID:
19464373
15.

Increase of soluble expression in Escherichia coli cytoplasm by a protein disulfide isomerase gene fusion system.

Liu Y, Zhao TJ, Yan YB, Zhou HM.

Protein Expr Purif. 2005 Dec;44(2):155-61. Epub 2005 Apr 19.

PMID:
15882951
16.
17.

Improving aquaporin Z expression in Escherichia coli by fusion partners and subsequent condition optimization.

Lian J, Ding S, Cai J, Zhang D, Xu Z, Wang X.

Appl Microbiol Biotechnol. 2009 Mar;82(3):463-70. doi: 10.1007/s00253-008-1774-x. Epub 2008 Nov 13.

PMID:
19005651
18.

A novel fusion protein system for the production of native human pepsinogen in the bacterial periplasm.

Malik A, Rudolph R, Söhling B.

Protein Expr Purif. 2006 Jun;47(2):662-71. Epub 2006 Mar 20.

PMID:
16600627
19.
20.

A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli.

Ashraf SS, Benson RE, Payne ES, Halbleib CM, Grøn H.

Protein Expr Purif. 2004 Feb;33(2):238-45.

PMID:
14711512

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