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Items: 1 to 20 of 86

1.

Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides.

Gibson DG.

Nucleic Acids Res. 2009 Nov;37(20):6984-90. doi: 10.1093/nar/gkp687. Epub 2009 Sep 10.

2.

Oligonucleotide assembly in yeast to produce synthetic DNA fragments.

Gibson DG.

Methods Mol Biol. 2012;852:11-21. doi: 10.1007/978-1-61779-564-0_2.

PMID:
22328422
3.

Assembling large DNA segments in yeast.

Muller H, Annaluru N, Schwerzmann JW, Richardson SM, Dymond JS, Cooper EM, Bader JS, Boeke JD, Chandrasegaran S.

Methods Mol Biol. 2012;852:133-50. doi: 10.1007/978-1-61779-564-0_11.

PMID:
22328431
4.

Gene2Oligo: oligonucleotide design for in vitro gene synthesis.

Rouillard JM, Lee W, Truan G, Gao X, Zhou X, Gulari E.

Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W176-80.

5.

Gene and genome construction in yeast.

Gibson DG.

Curr Protoc Mol Biol. 2011 Apr;Chapter 3:Unit3.22. doi: 10.1002/0471142727.mb0322s94.

PMID:
21472698
6.

Synthesis of a gene for human serum albumin and its expression in Saccharomyces cerevisiae.

Kálmán M, Cserpán I, Bajszár G, Dobi A, Horváth E, Pázmán C, Simoncsits A.

Nucleic Acids Res. 1990 Oct 25;18(20):6075-81.

7.

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.

Gibson DG, Benders GA, Axelrod KC, Zaveri J, Algire MA, Moodie M, Montague MG, Venter JC, Smith HO, Hutchison CA 3rd.

Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20404-9. doi: 10.1073/pnas.0811011106. Epub 2008 Dec 10.

8.

Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast.

Storici F, Durham CL, Gordenin DA, Resnick MA.

Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9. Epub 2003 Nov 20.

10.

Chemical synthesis of a mitochondrial gene designed for expression in the yeast nucleus.

Gearing DP, McMullen GL, Nagley P.

Biochem Int. 1985 Jun;10(6):907-15.

PMID:
2931082
11.

Oligonucleotide transformation of yeast reveals mismatch repair complexes to be differentially active on DNA replication strands.

Kow YW, Bao G, Reeves JW, Jinks-Robertson S, Crouse GF.

Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11352-7. Epub 2007 Jun 25.

12.

Parameters affecting the frequencies of transformation and co-transformation with synthetic oligonucleotides in yeast.

Yamamoto T, Moerschell RP, Wakem LP, Ferguson D, Sherman F.

Yeast. 1992 Nov;8(11):935-48.

PMID:
1336288
13.

Targeted deletions created in yeast vectors by recombinational excision.

Dunø M, Bendixen C, Krejci L, Thomsen B.

Nucleic Acids Res. 1999 Apr 15;27(8):e1.

14.

Oligonucleotide transformation for the study of mutagenic specificities of DNA lesions in yeast.

Negishi K, Sekine D, Morimitsu T, Suzuki T, Okugawa Y, Kawakami A, Otsuka C, Oyama H, Loakes D.

Nucleic Acids Symp Ser (Oxf). 2007;(51):211-2.

PMID:
18029661
15.

Transformation of yeast with synthetic oligonucleotides.

Moerschell RP, Tsunasawa S, Sherman F.

Proc Natl Acad Sci U S A. 1988 Jan;85(2):524-8.

16.

Total synthesis of multi-kilobase DNA sequences from oligonucleotides.

Reisinger SJ, Patel KG, Santi DV.

Nat Protoc. 2006;1(6):2596-603.

PMID:
17406514
17.
18.

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.

Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, Smith HO.

Science. 2008 Feb 29;319(5867):1215-20. doi: 10.1126/science.1151721. Epub 2008 Jan 24.

19.

Chemical synthesis, cloning and expression of human epidermal growth factor gene in Saccharomyces cerevisiae.

Yuan H, Min Y, Shi S, Tao W, Wu S, Li Y, Wang Q, Lu H, Zhang C, Hu Q.

Chin J Biotechnol. 1991;7(4):253-61.

PMID:
1668605
20.

Strand-specificity in the transformation of yeast with synthetic oligonucleotides.

Yamamoto T, Moerschell RP, Wakem LP, Komar-Panicucci S, Sherman F.

Genetics. 1992 Aug;131(4):811-9.

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