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Items: 1 to 20 of 133

1.

High quality protein microarray using in situ protein purification.

Kwon K, Grose C, Pieper R, Pandya GA, Fleischmann RD, Peterson SN.

BMC Biotechnol. 2009 Aug 23;9:72. doi: 10.1186/1472-6750-9-72.

2.

Purification of recombinant enhanced green fluorescent protein expressed in Escherichia coli with new immobilized metal ion affinity magnetic absorbents.

Chiang CL, Chen CY, Chang LW.

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15;864(1-2):116-22. doi: 10.1016/j.jchromb.2008.01.056. Epub 2008 Feb 20.

PMID:
18313994
3.

Use of streamline chelating for capture and purification of poly-His-tagged recombinant proteins.

Noronha S, Kaufman J, Shiloach J.

Bioseparation. 1999;8(1-5):145-51.

PMID:
10734566
4.

High-throughput purification of hexahistidine-tagged proteins expressed in E. coli.

Murphy MB, Doyle SA.

Methods Mol Biol. 2005;310:123-30.

PMID:
16350951
5.

Purification of a recombinant human growth hormone by an integrated IMAC procedure.

Mooney JT, Fredericks DP, Zhang C, Christensen T, Jespergaard C, Schiødt CB, Hearn MT.

Protein Expr Purif. 2014 Feb;94:85-94. doi: 10.1016/j.pep.2013.11.002. Epub 2013 Nov 22.

PMID:
24275639
6.

Facial synthesis of nickel(II)-immobilized carboxyl cotton chelator for purification of histidine-tagged proteins.

He XM, Yuan BF, Feng YQ.

J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Feb 1;1043:122-127. doi: 10.1016/j.jchromb.2016.06.029. Epub 2016 Jun 18.

PMID:
27372913
7.

High-throughput protein purification and quality assessment for crystallization.

Kim Y, Babnigg G, Jedrzejczak R, Eschenfeldt WH, Li H, Maltseva N, Hatzos-Skintges C, Gu M, Makowska-Grzyska M, Wu R, An H, Chhor G, Joachimiak A.

Methods. 2011 Sep;55(1):12-28. doi: 10.1016/j.ymeth.2011.07.010. Epub 2011 Aug 31. Review.

8.

Purification of His-Tagged Proteins.

Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B.

Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4.

PMID:
26096499
9.

Evaluation of immobilized metal affinity chromatography kits for the purification of histidine-tagged recombinant CagA protein.

Karakus C, Uslu M, Yazici D, Salih BA.

J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:182-7. doi: 10.1016/j.jchromb.2015.11.045. Epub 2015 Nov 26.

PMID:
26657801
10.

Immobilized palladium(II) ion affinity chromatography for recovery of recombinant proteins with peptide tags containing histidine and cysteine.

Kikot P, Polat A, Achilli E, Fernandez Lahore M, Grasselli M.

J Mol Recognit. 2014 Nov;27(11):659-68. doi: 10.1002/jmr.2389.

PMID:
25277090
11.

Changed loading conditions and lysate composition improve the purity of tagged recombinant proteins with tacn-based IMAC adsorbents.

Zhang C, Fredericks D, Longford D, Campi E, Sawford T, Hearn MT.

Biotechnol J. 2015 Mar;10(3):480-9. doi: 10.1002/biot.201400463. Epub 2014 Oct 31.

PMID:
25303209
12.

Nickel(II)-immobilized sulfhydryl cotton fiber for selective binding and rapid separation of histidine-tagged proteins.

He XM, Zhu GT, Lu W, Yuan BF, Wang H, Feng YQ.

J Chromatogr A. 2015 Jul 31;1405:188-92. doi: 10.1016/j.chroma.2015.05.040. Epub 2015 Jun 6.

PMID:
26087962
13.

His-tagged protein purification by metal-chelate affinity extraction with nickel-chelate reverse micelles.

Dong XY, Feng XD, Sun Y.

Biotechnol Prog. 2010 Jul-Aug;26(4):1088-94. doi: 10.1002/btpr.428.

PMID:
20730766
14.

Affinity chromatography of polyhistidine tagged enzymes. New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions.

Mateo C, Fernandez-Lorente G, Pessela BC, Vian A, Carrascosa AV, Garcia JL, Fernandez-Lafuente R, Guisan JM.

J Chromatogr A. 2001 Apr 27;915(1-2):97-106.

PMID:
11358266
15.

Orthogonal protein purification facilitated by a small bispecific affinity tag.

Nilvebrant J, Alm T, Hober S.

J Vis Exp. 2012 Jan 16;(59). pii: 3370. doi: 10.3791/3370.

16.
17.

Engineering Escherichia coli BL21(DE3) derivative strains to minimize E. coli protein contamination after purification by immobilized metal affinity chromatography.

Robichon C, Luo J, Causey TB, Benner JS, Samuelson JC.

Appl Environ Microbiol. 2011 Jul;77(13):4634-46. doi: 10.1128/AEM.00119-11. Epub 2011 May 20.

18.

Integrative refolding and purification of histidine-tagged protein by like-charge facilitated refolding and metal-chelate affinity adsorption.

Liu H, Du WJ, Dong XY, Sun Y.

J Chromatogr A. 2014 May 30;1344:59-65. doi: 10.1016/j.chroma.2014.04.006. Epub 2014 Apr 13.

PMID:
24768124
19.

Imidazole-free purification of His3-tagged recombinant proteins using ssDNA aptamer-based affinity chromatography.

Bartnicki F, Kowalska E, Pels K, Strzalka W.

J Chromatogr A. 2015 Oct 30;1418:130-9. doi: 10.1016/j.chroma.2015.09.055. Epub 2015 Sep 25.

PMID:
26427325
20.

Regioselective covalent immobilization of recombinant antibody-binding proteins A, G, and L for construction of antibody arrays.

Seo JS, Lee S, Poulter CD.

J Am Chem Soc. 2013 Jun 19;135(24):8973-80. doi: 10.1021/ja402447g. Epub 2013 Jun 10.

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