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Items: 1 to 20 of 77

1.

PCR-based methods for the enrichment of minority alleles and mutations.

Milbury CA, Li J, Makrigiorgos GM.

Clin Chem. 2009 Apr;55(4):632-40. doi: 10.1373/clinchem.2008.113035. Epub 2009 Feb 6. Review.

2.

Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.

Li J, Wang L, Jänne PA, Makrigiorgos GM.

Clin Chem. 2009 Apr;55(4):748-56. doi: 10.1373/clinchem.2008.113381. Epub 2009 Feb 20.

3.

COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations.

Milbury CA, Li J, Makrigiorgos GM.

Clin Chem. 2009 Dec;55(12):2130-43. doi: 10.1373/clinchem.2009.131029. Epub 2009 Oct 8.

4.

COLD-PCR enriches low-level variant DNA sequences and increases the sensitivity of genetic testing.

Castellanos-Rizaldos E, Milbury CA, Guha M, Makrigiorgos GM.

Methods Mol Biol. 2014;1102:623-39. doi: 10.1007/978-1-62703-727-3_33.

PMID:
24259002
5.

Enrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.

Castellanos-Rizaldos E, Milbury CA, Makrigiorgos GM.

PLoS One. 2012;7(12):e51362. doi: 10.1371/journal.pone.0051362. Epub 2012 Dec 6.

6.

Coamplification at lower denaturation temperature polymerase chain reaction enables selective identification of K-Ras mutations in formalin-fixed, paraffin-embedded tumor tissues without tumor-cell enrichment.

Yu S, Xie L, Hou Z, Qian X, Yu L, Wei J, Ding Y, Liu B.

Hum Pathol. 2011 Sep;42(9):1312-8. doi: 10.1016/j.humpath.2010.06.018. Epub 2011 Mar 15.

PMID:
21406308
7.

Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.

Milbury CA, Chen CC, Mamon H, Liu P, Santagata S, Makrigiorgos GM.

J Mol Diagn. 2011 Mar;13(2):220-32. doi: 10.1016/j.jmoldx.2010.10.008.

8.

COLD-PCR: improving the sensitivity of molecular diagnostics assays.

Milbury CA, Li J, Liu P, Makrigiorgos GM.

Expert Rev Mol Diagn. 2011 Mar;11(2):159-69. doi: 10.1586/erm.10.115.

9.

COLD-PCR: Applications and Advantages.

Zuo Z, Jabbar KJ.

Methods Mol Biol. 2016;1392:17-25. doi: 10.1007/978-1-4939-3360-0_2.

PMID:
26843042
10.

Enhanced ratio of signals enables digital mutation scanning for rare allele detection.

Castellanos-Rizaldos E, Paweletz C, Song C, Oxnard GR, Mamon H, Jänne PA, Makrigiorgos GM.

J Mol Diagn. 2015 May;17(3):284-92. doi: 10.1016/j.jmoldx.2014.12.003. Epub 2015 Mar 13.

11.

Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.

Milbury CA, Li J, Makrigiorgos GM.

Nucleic Acids Res. 2011 Jan;39(1):e2. doi: 10.1093/nar/gkq899. Epub 2010 Oct 11.

12.

Detection of hepatitis B virus genotypic resistance mutations by coamplification at lower denaturation temperature-PCR coupled with sanger sequencing.

Liu C, Lin J, Chen H, Shang H, Jiang L, Chen J, Ye Y, Yang B, Ou Q.

J Clin Microbiol. 2014 Aug;52(8):2933-9. doi: 10.1128/JCM.01127-14. Epub 2014 Jun 4.

13.

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.

Castellanos-Rizaldos E, Richardson K, Lin R, Wu G, Makrigiorgos MG.

Clin Chem. 2015 Jan;61(1):267-77. doi: 10.1373/clinchem.2014.228361. Epub 2014 Oct 8.

14.

Pyrosequencing®-Based Identification of Low-Frequency Mutations Enriched Through Enhanced-ice-COLD-PCR.

How-Kit A, Tost J.

Methods Mol Biol. 2015;1315:83-101. doi: 10.1007/978-1-4939-2715-9_7.

PMID:
26103893
15.

COLD-PCR and innovative microarray substrates for detecting and genotyping MPL exon 10 W515 substitutions.

Brisci A, Damin F, Pietra D, Galbiati S, Boggi S, Casetti I, Rumi E, Chiari M, Cazzola M, Ferrari M, Cremonesi L.

Clin Chem. 2012 Dec;58(12):1692-702. doi: 10.1373/clinchem.2012.192708. Epub 2012 Oct 11.

16.

BRAFV600E detection in melanoma is highly improved by COLD-PCR.

Pinzani P, Santucci C, Mancini I, Simi L, Salvianti F, Pratesi N, Massi D, De Giorgi V, Pazzagli M, Orlando C.

Clin Chim Acta. 2011 May 12;412(11-12):901-5. doi: 10.1016/j.cca.2011.01.014. Epub 2011 Jan 22.

PMID:
21262211
17.

Combining COLD-PCR and high-resolution melt analysis for rapid detection of low-level, rifampin-resistant mutations in Mycobacterium tuberculosis.

Pang Y, Liu G, Wang Y, Zheng S, Zhao YL.

J Microbiol Methods. 2013 Apr;93(1):32-6. doi: 10.1016/j.mimet.2013.01.008. Epub 2013 Feb 8.

PMID:
23396215
18.

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.

Li J, Makrigiorgos GM.

Biochem Soc Trans. 2009 Apr;37(Pt 2):427-32. doi: 10.1042/BST0370427. Review.

PMID:
19290875
19.

Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens.

Dominguez PL, Kolodney MS.

Oncogene. 2005 Oct 13;24(45):6830-4. Erratum in: Oncogene. 2006 Jan 26;25(4):656.

PMID:
16116485
20.

PCR-based detection of minority point mutations.

Mike Makrigiorgos G.

Hum Mutat. 2004 May;23(5):406-12. Review.

PMID:
15108270

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