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Use of the viral 2A peptide for bicistronic expression in transgenic mice.

Trichas G, Begbie J, Srinivas S.

BMC Biol. 2008 Sep 15;6:40. doi: 10.1186/1741-7007-6-40.


Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system.

Sun H, Zhou N, Wang H, Huang D, Lang Z.

PLoS One. 2017 Mar 30;12(3):e0174804. doi: 10.1371/journal.pone.0174804. eCollection 2017.


High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice.

Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, Choi SY.

PLoS One. 2011;6(4):e18556. doi: 10.1371/journal.pone.0018556. Epub 2011 Apr 29.


Highly efficient multicistronic lentiviral vectors with peptide 2A sequences.

Ibrahimi A, Vande Velde G, Reumers V, Toelen J, Thiry I, Vandeputte C, Vets S, Deroose C, Bormans G, Baekelandt V, Debyser Z, Gijsbers R.

Hum Gene Ther. 2009 Aug;20(8):845-60. doi: 10.1089/hum.2008.188.


Bicistronic lentiviruses containing a viral 2A cleavage sequence reliably co-express two proteins and restore vision to an animal model of LCA1.

Verrier JD, Madorsky I, Coggin WE, Geesey M, Hochman M, Walling E, Daroszewski D, Eccles KS, Ludlow R, Semple-Rowland SL.

PLoS One. 2011;6(5):e20553. doi: 10.1371/journal.pone.0020553. Epub 2011 May 27.


A novel bicistronic expression system composed of the intraflagellar transport protein gene ift25 and FMDV 2A sequence directs robust nuclear gene expression in Chlamydomonas reinhardtii.

Dong B, Hu HH, Li ZF, Cheng RQ, Meng DM, Wang J, Fan ZC.

Appl Microbiol Biotechnol. 2017 May;101(10):4227-4245. doi: 10.1007/s00253-017-8177-9. Epub 2017 Feb 25.


Expression of multiple transgenes from a single construct using viral 2A peptides in Drosophila.

Daniels RW, Rossano AJ, Macleod GT, Ganetzky B.

PLoS One. 2014 Jun 19;9(6):e100637. doi: 10.1371/journal.pone.0100637. eCollection 2014.


Afp::mCherry, a red fluorescent transgenic reporter of the mouse visceral endoderm.

Viotti M, Nowotschin S, Hadjantonakis AK.

Genesis. 2011 Mar;49(3):124-33. doi: 10.1002/dvg.20695. Epub 2011 Jan 5.


Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI.

Chinnasamy D, Milsom MD, Shaffer J, Neuenfeldt J, Shaaban AF, Margison GP, Fairbairn LJ, Chinnasamy N.

Virol J. 2006 Mar 15;3:14.


GFAP promoter drives Müller cell-specific expression in transgenic mice.

Kuzmanovic M, Dudley VJ, Sarthy VP.

Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3606-13.


Transgene expression of green fluorescent protein and germ line transmission in cloned calves derived from in vitro-transfected somatic cells.

Bordignon V, Keyston R, Lazaris A, Bilodeau AS, Pontes JH, Arnold D, Fecteau G, Keefer C, Smith LC.

Biol Reprod. 2003 Jun;68(6):2013-23. Epub 2003 Jan 8.


Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.

Mort RL, Ford MJ, Sakaue-Sawano A, Lindstrom NO, Casadio A, Douglas AT, Keighren MA, Hohenstein P, Miyawaki A, Jackson IJ.

Cell Cycle. 2014;13(17):2681-96. doi: 10.4161/15384101.2015.945381.


Effect of the Emu IgH enhancer on expression of a GFP reporter gene in transfected B cells and transgenic mice.

Guglielmi L, Le Bert M, Cogné M, Denizot Y.

Immunol Lett. 2003 Mar 3;86(1):77-83.


The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo.

Lewis JE, Brameld JM, Hill P, Barrett P, Ebling FJ, Jethwa PH.

J Neurosci Methods. 2015 Dec 30;256:22-9. doi: 10.1016/j.jneumeth.2015.08.013. Epub 2015 Aug 20.

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