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Items: 1 to 20 of 98

1.

Cloning, purification and initial characterization of E. coli McrA, a putative 5-methylcytosine-specific nuclease.

Mulligan EA, Dunn JJ.

Protein Expr Purif. 2008 Nov;62(1):98-103. doi: 10.1016/j.pep.2008.06.016. Epub 2008 Jul 10.

2.

Differential binding of Escherichia coli McrA protein to DNA sequences that contain the dinucleotide m5CpG.

Mulligan EA, Hatchwell E, McCorkle SR, Dunn JJ.

Nucleic Acids Res. 2010 Apr;38(6):1997-2005. doi: 10.1093/nar/gkp1120. Epub 2009 Dec 16.

3.
4.

Modified-cytosine restriction-system-induced recombinant cloning artefacts in Escherichia coli.

Williamson MR, Doherty JP, Woodcock DM.

Gene. 1993 Feb 14;124(1):37-44.

PMID:
8382656
5.

McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.

Raleigh EA, Murray NE, Revel H, Blumenthal RM, Westaway D, Reith AD, Rigby PW, Elhai J, Hanahan D.

Nucleic Acids Res. 1988 Feb 25;16(4):1563-75. Erratum in: Nucleic Acids Res 1995 Sep 11;23(17):3612.

6.

Engineering Escherichia coli BL21(DE3) derivative strains to minimize E. coli protein contamination after purification by immobilized metal affinity chromatography.

Robichon C, Luo J, Causey TB, Benner JS, Samuelson JC.

Appl Environ Microbiol. 2011 Jul;77(13):4634-46. doi: 10.1128/AEM.00119-11. Epub 2011 May 20.

7.

High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli.

Chen J, Li Y, Wang E, Wang Y.

Protein Expr Purif. 1999 Feb;15(1):115-20.

PMID:
10024478
8.

Cloning and characterization of the HpaII methylase gene.

Card CO, Wilson GG, Weule K, Hasapes J, Kiss A, Roberts RJ.

Nucleic Acids Res. 1990 Mar 25;18(6):1377-83.

9.

Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

Raleigh EA, Wilson G.

Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070-4.

10.
11.

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

Fomenkov A, Xiao JP, Dila D, Raleigh E, Xu SY.

Nucleic Acids Res. 1994 Jun 25;22(12):2399-403.

12.
13.

Overexpression, purification, and characterization of recombinant T4 gene 32 protein22-301 (g32P-B).

Giedroc DP, Khan R, Barnhart K.

J Biol Chem. 1990 Jul 15;265(20):11444-55.

14.

[Influence of the reductase deficient Escherichia coli on the solubility of recombinant proteins produced in it].

Xiong S, Zhang MY, Qian CW, Ran YC, Wang YF, Ren XR, Su KY, Yu ZY.

Sheng Wu Gong Cheng Xue Bao. 2003 Nov;19(6):686-91. Chinese.

PMID:
15971580
17.

Expression of biologically active human SPARC in Escherichia coli.

Bassuk JA, Baneyx F, Vernon RB, Funk SE, Sage EH.

Arch Biochem Biophys. 1996 Jan 1;325(1):8-19.

PMID:
8554346
18.

Expression and purification of histidine-tagged bacteriophage T7 DNA polymerase.

Schlicke M, Brakmann S.

Protein Expr Purif. 2005 Feb;39(2):247-53.

PMID:
15642476
19.

[Optimization of expression and purification of recombinant Salvia miltiorrhiza WRKY1 protein in Escherichia coli].

Liu YZ, Shen Y, Rong QX, Wu WY, Li RB, Wu ZG, Chen M.

Zhongguo Zhong Yao Za Zhi. 2014 Apr;39(7):1214-9. Chinese.

PMID:
25011256

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