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Items: 1 to 20 of 130

1.

Thermostable Bacillus subtilis lipases: in vitro evolution and structural insight.

Ahmad S, Kamal MZ, Sankaranarayanan R, Rao NM.

J Mol Biol. 2008 Aug 29;381(2):324-40. doi: 10.1016/j.jmb.2008.05.063. Epub 2008 Jul 2.

PMID:
18599073
2.

Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase.

Acharya P, Rajakumara E, Sankaranarayanan R, Rao NM.

J Mol Biol. 2004 Aug 27;341(5):1271-81.

PMID:
15321721
3.

Crystallization and preliminary X-ray crystallographic investigations on several thermostable forms of a Bacillus subtilis lipase.

Rajakumara E, Acharya P, Ahmad S, Shanmugam VM, Rao NM, Sankaranarayanan R.

Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):160-2. Epub 2003 Dec 18.

PMID:
14684916
4.

Stability curves of laboratory evolved thermostable mutants of a Bacillus subtilis lipase.

Kamal MZ, Ahmad S, Yedavalli P, Rao NM.

Biochim Biophys Acta. 2010 Sep;1804(9):1850-6. doi: 10.1016/j.bbapap.2010.06.014. Epub 2010 Jun 25.

PMID:
20599630
5.

In vitro evolved non-aggregating and thermostable lipase: structural and thermodynamic investigation.

Kamal MZ, Ahmad S, Molugu TR, Vijayalakshmi A, Deshmukh MV, Sankaranarayanan R, Rao NM.

J Mol Biol. 2011 Oct 28;413(3):726-41. doi: 10.1016/j.jmb.2011.09.002. Epub 2011 Sep 10.

PMID:
21925508
6.

Mutatomics analysis of the systematic thermostability profile of Bacillus subtilis lipase A.

Tian F, Yang C, Wang C, Guo T, Zhou P.

J Mol Model. 2014 Jun;20(6):2257. doi: 10.1007/s00894-014-2257-x. Epub 2014 May 15.

PMID:
24827611
7.

Engineering lipase A from mesophilic Bacillus subtilis for activity at low temperatures.

Kumar V, Yedavalli P, Gupta V, Rao NM.

Protein Eng Des Sel. 2014 Mar;27(3):73-82. doi: 10.1093/protein/gzt064. Epub 2014 Jan 8.

PMID:
24402332
8.

Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention.

Augustyniak W, Brzezinska AA, Pijning T, Wienk H, Boelens R, Dijkstra BW, Reetz MT.

Protein Sci. 2012 Apr;21(4):487-97. doi: 10.1002/pro.2031. Epub 2012 Feb 29.

9.

Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH.

Rajakumara E, Acharya P, Ahmad S, Sankaranaryanan R, Rao NM.

Biochim Biophys Acta. 2008 Feb;1784(2):302-11. Epub 2007 Nov 12.

PMID:
18053819
10.

Understanding the thermostability and activity of Bacillus subtilis lipase mutants: insights from molecular dynamics simulations.

Singh B, Bulusu G, Mitra A.

J Phys Chem B. 2015 Jan 15;119(2):392-409. doi: 10.1021/jp5079554. Epub 2015 Jan 2.

PMID:
25495458
11.
12.

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability.

Reetz MT, Carballeira JD, Vogel A.

Angew Chem Int Ed Engl. 2006 Nov 27;45(46):7745-51. No abstract available.

PMID:
17075931
13.

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes.

Reetz MT, Carballeira JD.

Nat Protoc. 2007;2(4):891-903.

PMID:
17446890
14.

Point mutation Gln121-Arg increased temperature optima of Bacillus lipase (1.4 subfamily) by fifteen degrees.

Goomber S, Kumar R, Singh R, Mishra N, Kaur J.

Int J Biol Macromol. 2016 Jul;88:507-14. doi: 10.1016/j.ijbiomac.2016.04.022. Epub 2016 Apr 13.

PMID:
27083848
15.
16.

In-vitro selection of highly stabilized protein variants with optimized surface.

Martin A, Sieber V, Schmid FX.

J Mol Biol. 2001 Jun 8;309(3):717-26.

PMID:
11397091
17.
18.

Optimized variants of the cold shock protein from in vitro selection: structural basis of their high thermostability.

Max KE, Wunderlich M, Roske Y, Schmid FX, Heinemann U.

J Mol Biol. 2007 Jun 15;369(4):1087-97. Epub 2007 Apr 12.

PMID:
17481655
19.

Just an additional hydrogen bond can dramatically reduce the catalytic activity of Bacillus subtilis lipase A I12T mutant: an integration of computational modeling and experimental analysis.

Ni Z, Jin R, Chen H, Lin X.

Comput Biol Med. 2013 Nov;43(11):1882-8. doi: 10.1016/j.compbiomed.2013.08.018. Epub 2013 Sep 13.

PMID:
24209933
20.

Probing protein stability and proteolytic resistance by loop scanning: a comprehensive mutational analysis.

Ahmad S, Kumar V, Ramanand KB, Rao NM.

Protein Sci. 2012 Mar;21(3):433-46. doi: 10.1002/pro.2029. Epub 2012 Feb 6.

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