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Items: 1 to 20 of 188

1.

The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12.

Baba T, Mori H.

Methods Mol Biol. 2008;416:171-81. doi: 10.1007/978-1-59745-321-9_11.

PMID:
18392967
2.

The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12.

Baba T, Huan HC, Datsenko K, Wanner BL, Mori H.

Methods Mol Biol. 2008;416:183-94. doi: 10.1007/978-1-59745-321-9_12.

PMID:
18392968
4.

Efficient and precise construction of markerless manipulations in the Bacillus subtilis genome.

Yu H, Yan X, Shen W, Shen Y, Zhang J, Li S.

J Microbiol Biotechnol. 2010 Jan;20(1):45-53.

5.

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.

Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H.

Mol Syst Biol. 2006;2:2006.0008. Epub 2006 Feb 21.

6.

PCR-mediated gene replacement in Escherichia coli.

Murphy KC, Campellone KG, Poteete AR.

Gene. 2000 Apr 4;246(1-2):321-30.

PMID:
10767554
7.

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Datsenko KA, Wanner BL.

Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5.

8.

Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.

Tischer BK, von Einem J, Kaufer B, Osterrieder N.

Biotechniques. 2006 Feb;40(2):191-7.

9.

Excisable cassettes: new tools for functional analysis of Streptomyces genomes.

Raynal A, Karray F, Tuphile K, Darbon-Rongère E, Pernodet JL.

Appl Environ Microbiol. 2006 Jul;72(7):4839-44.

10.

DelsGate, a robust and rapid gene deletion construction method.

García-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE.

Fungal Genet Biol. 2008 Apr;45(4):379-88. doi: 10.1016/j.fgb.2007.11.001. Epub 2007 Nov 19.

PMID:
18248826
11.

Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes.

Studier FW, Daegelen P, Lenski RE, Maslov S, Kim JF.

J Mol Biol. 2009 Dec 11;394(4):653-80. doi: 10.1016/j.jmb.2009.09.021. Epub 2009 Sep 15.

PMID:
19765592
12.

Rapid allelic exchange in enterohemorrhagic Escherichia coli (EHEC) and other E. coli using lambda red recombination.

Savage PJ, Leong JM, Murphy KC.

Curr Protoc Microbiol. 2006 Jan;Chapter 5:Unit5A.2. doi: 10.1002/9780471729259.mc05a02s00.

PMID:
18770591
13.

Rapid and efficient construction of markerless deletions in the Escherichia coli genome.

Yu BJ, Kang KH, Lee JH, Sung BH, Kim MS, Kim SC.

Nucleic Acids Res. 2008 Aug;36(14):e84. doi: 10.1093/nar/gkn359. Epub 2008 Jun 21.

14.

Scarless engineering of the Escherichia coli genome.

Fehér T, Karcagi I, Gyorfy Z, Umenhoffer K, Csörgo B, Pósfai G.

Methods Mol Biol. 2008;416:251-9. doi: 10.1007/978-1-59745-321-9_16.

PMID:
18392972
15.

Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system.

Yu BJ, Kim C.

Methods Mol Biol. 2008;416:261-77. doi: 10.1007/978-1-59745-321-9_17.

PMID:
18392973
16.

Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system.

Yu BJ, Sung BH, Koob MD, Lee CH, Lee JH, Lee WS, Kim MS, Kim SC.

Nat Biotechnol. 2002 Oct;20(10):1018-23. Epub 2002 Sep 16.

PMID:
12244329
18.
19.

A new simple method to introduce marker-free deletions in the Bacillus subtilis genome.

Morimoto T, Ara K, Ozaki K, Ogasawara N.

Genes Genet Syst. 2009 Aug;84(4):315-8.

20.

Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome.

Kato J, Hashimoto M.

Methods Mol Biol. 2008;416:279-93. doi: 10.1007/978-1-59745-321-9_18.

PMID:
18392974

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