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Items: 1 to 20 of 85

1.

Increase of calnexin gene dosage boosts the secretion of heterologous proteins by Hansenula polymorpha.

Klabunde J, Kleebank S, Piontek M, Hollenberg CP, Hellwig S, Degelmann A.

FEMS Yeast Res. 2007 Oct;7(7):1168-80. Epub 2007 Jul 6.

2.

Pphy--a cell-bound phytase from the yeast Pichia anomala: molecular cloning of the gene PPHY and characterization of the recombinant enzyme.

Kaur P, Singh B, Böer E, Straube N, Piontek M, Satyanarayana T, Kunze G.

J Biotechnol. 2010 Aug 20;149(1-2):8-15. doi: 10.1016/j.jbiotec.2010.06.017. Epub 2010 Jun 25.

PMID:
20599570
3.

Xplor 2--an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans.

Böer E, Piontek M, Kunze G.

Appl Microbiol Biotechnol. 2009 Sep;84(3):583-94. doi: 10.1007/s00253-009-2167-5. Epub 2009 Aug 12.

PMID:
19672589
4.

Characterization of N-linked oligosaccharides assembled on secretory recombinant glucose oxidase and cell wall mannoproteins from the methylotrophic yeast Hansenula polymorpha.

Kim MW, Rhee SK, Kim JY, Shimma Y, Chiba Y, Jigami Y, Kang HA.

Glycobiology. 2004 Mar;14(3):243-51. Epub 2003 Dec 23.

PMID:
14693910
5.

Enhancement of alkaline phytase production in Pichia pastoris: influence of gene dosage, sequence optimization and expression temperature.

Yang M, Johnson SC, Murthy PP.

Protein Expr Purif. 2012 Aug;84(2):247-54. doi: 10.1016/j.pep.2012.06.001. Epub 2012 Jun 15.

PMID:
22705766
6.

Functional characterization of the Hansenula polymorpha HOC1, OCH1, and OCR1 genes as members of the yeast OCH1 mannosyltransferase family involved in protein glycosylation.

Kim MW, Kim EJ, Kim JY, Park JS, Oh DB, Shimma Y, Chiba Y, Jigami Y, Rhee SK, Kang HA.

J Biol Chem. 2006 Mar 10;281(10):6261-72. Epub 2006 Jan 10.

7.

Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMA1 promoter.

Cox H, Mead D, Sudbery P, Eland RM, Mannazzu I, Evans L.

Yeast. 2000 Sep 30;16(13):1191-203.

8.

Expression of bovine follicle-stimulating hormone subunits in a Hansenula polymorpha expression system increases the secretion and bioactivity in vivo.

Qian W, Liu Y, Zhang C, Niu Z, Song H, Qiu B.

Protein Expr Purif. 2009 Dec;68(2):183-9. doi: 10.1016/j.pep.2009.07.008. Epub 2009 Jul 25.

PMID:
19635568
9.

Identification and functional characterization of the HpALG11 and the HpRFT1 genes involved in N-linked glycosylation in the methylotrophic yeast Hansenula polymorpha.

Song H, Qian W, Wang H, Qiu B.

Glycobiology. 2010 Dec;20(12):1665-74. doi: 10.1093/glycob/cwq121. Epub 2010 Aug 9.

PMID:
20696712
10.

Expression of the alpha-galactosidase from Cyamopsis tetragonoloba (guar) by Hansenula polymorpha.

Fellinger AJ, Verbakel JM, Veale RA, Sudbery PE, Bom IJ, Overbeeke N, Verrips CT.

Yeast. 1991 Jul;7(5):463-73.

PMID:
1654681
11.

Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae MNN9 gene.

Kim SY, Sohn JH, Kang HA, Yoo SK, Pyun YR, Choi ES.

Yeast. 2001 Mar 30;18(5):455-61.

12.

Mutation of the protein-O-mannosyltransferase enhances secretion of the human urokinase-type plasminogen activator in Hansenula polymorpha.

Agaphonov MO, Sokolov SS, Romanova NV, Sohn JH, Kim SY, Kalebina TS, Choi ES, Ter-Avanesyan MD.

Yeast. 2005 Oct 15;22(13):1037-47.

13.

Single-step co-integration of multiple expressible heterologous genes into the ribosomal DNA of the methylotrophic yeast Hansenula polymorpha.

Klabunde J, Diesel A, Waschk D, Gellissen G, Hollenberg CP, Suckow M.

Appl Microbiol Biotechnol. 2002 May;58(6):797-805. Epub 2002 Mar 19.

PMID:
12021801
14.

Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase.

Gellissen G, Janowicz ZA, Merckelbach A, Piontek M, Keup P, Weydemann U, Hollenberg CP, Strasser AW.

Biotechnology (N Y). 1991 Mar;9(3):291-5.

PMID:
1367303
15.
16.

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

Martínez-Ruiz A, Martínez del Pozo A, Lacadena J, Mancheño JM, Oñaderra M, López-otín C, Gavilanes JG.

Protein Expr Purif. 1998 Apr;12(3):315-22.

PMID:
9535698
17.

Comparison of two expression platforms in respect to protein yield and quality: Pichia pastoris versus Pichia angusta.

Mack M, Wannemacher M, Hobl B, Pietschmann P, Hock B.

Protein Expr Purif. 2009 Aug;66(2):165-71. doi: 10.1016/j.pep.2009.03.010. Epub 2009 Apr 7.

PMID:
19358890
18.

Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene.

Gidijala L, van der Klei IJ, Veenhuis M, Kiel JA.

FEMS Yeast Res. 2007 Oct;7(7):1160-7. Epub 2007 Apr 10.

19.

Isolation, characterization, and molecular cloning of the cDNA encoding a novel phytase from Aspergillus niger 113 and high expression in Pichia pastoris.

Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Guo MJ, Zhang SL.

J Biochem Mol Biol. 2004 May 31;37(3):282-91.

PMID:
15469708
20.

Molecular gene cloning and overexpression of the phytase from Debaryomyces castellii CBS 2923.

Ragon M, Neugnot-Roux V, Chemardin P, Moulin G, Boze H.

Protein Expr Purif. 2008 Apr;58(2):275-83. doi: 10.1016/j.pep.2007.12.003. Epub 2008 Jan 10.

PMID:
18242101

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