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Items: 1 to 20 of 112

1.

Immobilized pH gradient isoelectric focusing as a first-dimension separation in shotgun proteomics.

Cargile BJ, Sevinsky JR, Essader AS, Stephenson JL Jr, Bundy JL.

J Biomol Tech. 2005 Sep;16(3):181-9. Review.

2.
3.

Shotgun proteomics: a qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS.

Geiser L, Vaezzadeh AR, Deshusses JM, Hochstrasser DF.

Methods Mol Biol. 2011;681:449-58. doi: 10.1007/978-1-60761-913-0_26.

PMID:
20978982
4.

Characterization of the rat liver membrane proteome using peptide immobilized pH gradient isoelectric focusing.

Chick JM, Haynes PA, Molloy MP, Bjellqvist B, Baker MS, Len AC.

J Proteome Res. 2008 Mar;7(3):1036-45. doi: 10.1021/pr700611w. Epub 2008 Jan 23.

PMID:
18211008
5.
6.

Observed peptide pI and retention time shifts as a result of post-translational modifications in multidimensional separations using narrow-range IPG-IEF.

Lengqvist J, Eriksson H, Gry M, Uhlén K, Björklund C, Bjellqvist B, Jakobsson PJ, Lehtiö J.

Amino Acids. 2011 Feb;40(2):697-711. doi: 10.1007/s00726-010-0704-2. Epub 2010 Aug 20.

PMID:
20725754
7.

Evaluation of strong cation exchange versus isoelectric focusing of peptides for multidimensional liquid chromatography-tandem mass spectrometry.

Slebos RJ, Brock JW, Winters NF, Stuart SR, Martinez MA, Li M, Chambers MC, Zimmerman LJ, Ham AJ, Tabb DL, Liebler DC.

J Proteome Res. 2008 Dec;7(12):5286-94. doi: 10.1021/pr8004666.

8.

iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing.

Lengqvist J, Uhlén K, Lehtiö J.

Proteomics. 2007 Jun;7(11):1746-52.

PMID:
17476709
9.

Proteome analysis using isoelectric focusing in immobilized pH gradient gels followed by mass spectrometry.

Giorgianni F, Desiderio DM, Beranova-Giorgianni S.

Electrophoresis. 2003 Jan;24(1-2):253-9.

PMID:
12652597
10.

Two-dimensional gel isoelectric focusing.

Stastná M, Slais K.

Electrophoresis. 2005 Sep;26(18):3586-91.

PMID:
16100746
11.

Narrow-range peptide isoelectric focusing as peptide prefractionation method prior to tandem mass spectrometry analysis.

Pernemalm M.

Methods Mol Biol. 2013;1023:3-11. doi: 10.1007/978-1-4614-7209-4_1.

PMID:
23765616
12.
13.

Imaging mass spectrometry using peptide isoelectric focusing.

Vaezzadeh AR, Simicevic J, Chauvet A, François P, Zimmermann-Ivol CG, Lescuyer P, Deshusses JP, Hochstrasser DF.

Rapid Commun Mass Spectrom. 2008 Sep;22(17):2667-76. doi: 10.1002/rcm.3658.

PMID:
18677718
14.

Membrane-assisted isoelectric focusing device as a micropreparative fractionator for two-dimensional shotgun proteomics.

Pirmoradian M, Zhang B, Chingin K, Astorga-Wells J, Zubarev RA.

Anal Chem. 2014 Jun 17;86(12):5728-32. doi: 10.1021/ac404180e. Epub 2014 May 28.

PMID:
24824042
15.

Isoelectric focusing in long immobilized pH gradient gels to improve protein separation in proteomic analysis.

Poland J, Cahill MA, Sinha P.

Electrophoresis. 2003 Apr;24(7-8):1271-5.

PMID:
12707921
16.

IPG strip-based peptide fractionation for shotgun proteomics.

Eravci M, Sommer C, Selbach M.

Methods Mol Biol. 2014;1156:67-77. doi: 10.1007/978-1-4939-0685-7_5.

PMID:
24791982
17.

A combination of immobilised pH gradients improves membrane proteomics.

Chick JM, Haynes PA, Bjellqvist B, Baker MS.

J Proteome Res. 2008 Nov;7(11):4974-81. doi: 10.1021/pr800349f. Epub 2008 Oct 7.

PMID:
18837535
18.

Added value for tandem mass spectrometry shotgun proteomics data validation through isoelectric focusing of peptides.

Heller M, Ye M, Michel PE, Morier P, Stalder D, Jünger MA, Aebersold R, Reymond F, Rossier JS.

J Proteome Res. 2005 Nov-Dec;4(6):2273-82.

PMID:
16335976
19.

No peptide left behind: the "out of range" recovery in IPG-IEF fractionation.

Iori E, Rattazzi M, Millioni R.

Amino Acids. 2014 May;46(5):1415-7. doi: 10.1007/s00726-014-1720-4. Epub 2014 Mar 11.

PMID:
24615240
20.

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