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Items: 1 to 20 of 116

1.

Complete, gene-specific siRNA libraries: production and expression in mammalian cells.

Seyhan AA, Vlassov AV, Ilves H, Egry L, Kaspar RL, Kazakov SA, Johnston BH.

RNA. 2005 May;11(5):837-46.

2.

Small interfering RNA production by enzymatic engineering of DNA (SPEED).

Luo B, Heard AD, Lodish HF.

Proc Natl Acad Sci U S A. 2004 Apr 13;101(15):5494-9. Epub 2004 Mar 15.

3.

Expressing functional siRNAs in mammalian cells using convergent transcription.

Tran N, Cairns MJ, Dawes IW, Arndt GM.

BMC Biotechnol. 2003 Nov 6;3:21.

4.

A simplified and versatile system for the simultaneous expression of multiple siRNAs in mammalian cells using Gibson DNA Assembly.

Deng F, Chen X, Liao Z, Yan Z, Wang Z, Deng Y, Zhang Q, Zhang Z, Ye J, Qiao M, Li R, Denduluri S, Wang J, Wei Q, Li M, Geng N, Zhao L, Zhou G, Zhang P, Luu HH, Haydon RC, Reid RR, Yang T, He TC.

PLoS One. 2014 Nov 14;9(11):e113064. doi: 10.1371/journal.pone.0113064. eCollection 2014.

5.

Optimization of linear double-stranded RNA for the production of multiple siRNAs targeting hepatitis C virus.

Shin D, Lee H, Kim SI, Yoon Y, Kim M.

RNA. 2009 May;15(5):898-910. doi: 10.1261/rna.1268209. Epub 2009 Mar 26.

6.

[Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene].

Luo H, Hu DX, Chen P.

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Jun;32(3):437-42. Chinese.

7.
8.

Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cells.

Cheng TL, Chang WT.

Methods Mol Biol. 2007;408:223-41. doi: 10.1007/978-1-59745-547-3_13.

PMID:
18314586
9.

Screening of siRNA target sequences by using fragmentized DNA.

Kasim V, Taira K, Miyagishi M.

J Gene Med. 2006 Jun;8(6):782-91.

PMID:
16532512
10.

RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells.

Yu JY, DeRuiter SL, Turner DL.

Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6047-52. Epub 2002 Apr 23.

11.

Rational design and in vitro and in vivo delivery of Dicer substrate siRNA.

Amarzguioui M, Lundberg P, Cantin E, Hagstrom J, Behlke MA, Rossi JJ.

Nat Protoc. 2006;1(2):508-17.

PMID:
17406276
12.

An approach to genomewide screens of expressed small interfering RNAs in mammalian cells.

Zheng L, Liu J, Batalov S, Zhou D, Orth A, Ding S, Schultz PG.

Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):135-40. Epub 2003 Dec 19.

13.
14.

MISSION esiRNA for RNAi screening in mammalian cells.

Theis M, Buchholz F.

J Vis Exp. 2010 May 12;(39). pii: 2008. doi: 10.3791/2008.

15.
16.

RNA interference (RNAi) with RNase III-prepared siRNAs.

Yang D, Goga A, Bishop JM.

Methods Mol Biol. 2004;252:471-82.

PMID:
15017072
17.

High-throughput screening of effective siRNAs using luciferase-linked chimeric mRNA.

Pang S, Pokomo L, Chen K, Kamata M, Mao SH, Zhang H, Razi E, An DS, Chen IS.

PLoS One. 2014 May 15;9(5):e96445. doi: 10.1371/journal.pone.0096445. eCollection 2014.

18.

siRNA-like double-stranded RNAs are specifically protected against degradation in human cell extract.

Hoerter JA, Krishnan V, Lionberger TA, Walter NG.

PLoS One. 2011;6(5):e20359. doi: 10.1371/journal.pone.0020359. Epub 2011 May 27.

19.

Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing.

Myers JW, Jones JT, Meyer T, Ferrell JE Jr.

Nat Biotechnol. 2003 Mar;21(3):324-8. Epub 2003 Feb 18.

PMID:
12592410
20.

Construction and transfection of PCR products expressing siRNAs or shRNAs in mammalian cells.

Castanotto D, Rossi JJ.

Methods Mol Biol. 2004;252:509-14.

PMID:
15017076

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