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Items: 1 to 20 of 96

1.

Development of a fluorescence polarization bead-based coupled assay to target different activity/conformation states of a protein kinase.

Lu Z, Yin Z, James L, Syto R, Stafford JM, Koseoglu S, Mayhood T, Myers J, Windsor W, Kirschmeier P, Samatar AA, Malcolm B, Turek-Etienne TC, Kumar CC.

J Biomol Screen. 2004 Jun;9(4):309-21.

PMID:
15191648
2.

A high-throughput, nonisotopic, competitive binding assay for kinases using nonselective inhibitor probes (ED-NSIP).

Vainshtein I, Silveria S, Kaul P, Rouhani R, Eglen RM, Wang J.

J Biomol Screen. 2002 Dec;7(6):507-14.

PMID:
14599348
3.

Development of a fluorescence polarization AKT serine/threonine kinase assay using an immobilized metal ion affinity-based technology.

Turek-Etienne TC, Kober TP, Stafford JM, Bryant RW.

Assay Drug Dev Technol. 2003 Aug;1(4):545-53.

PMID:
15090251
4.
5.

Phosphoinositide-dependent protein kinase 1, a sensor of protein conformation.

Biondi RM.

Trends Biochem Sci. 2004 Mar;29(3):136-42. Review.

PMID:
15003271
7.
8.

Cyclic AMP inhibits Akt activity by blocking the membrane localization of PDK1.

Kim S, Jee K, Kim D, Koh H, Chung J.

J Biol Chem. 2001 Apr 20;276(16):12864-70.

9.

A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation.

Frödin M, Antal TL, Dümmler BA, Jensen CJ, Deak M, Gammeltoft S, Biondi RM.

EMBO J. 2002 Oct 15;21(20):5396-407.

10.
11.

Discovery of PDK1 kinase inhibitors with a novel mechanism of action by ultrahigh throughput screening.

Bobkova EV, Weber MJ, Xu Z, Zhang YL, Jung J, Blume-Jensen P, Northrup A, Kunapuli P, Andersen JN, Kariv I.

J Biol Chem. 2010 Jun 11;285(24):18838-46. doi: 10.1074/jbc.M109.089946.

12.

Insulin-stimulated protein kinase B phosphorylation on Ser-473 is independent of its activity and occurs through a staurosporine-insensitive kinase.

Hill MM, Andjelkovic M, Brazil DP, Ferrari S, Fabbro D, Hemmings BA.

J Biol Chem. 2001 Jul 13;276(28):25643-6.

13.

In vivo role of the phosphate groove of PDK1 defined by knockin mutation.

Collins BJ, Deak M, Murray-Tait V, Storey KG, Alessi DR.

J Cell Sci. 2005 Nov 1;118(Pt 21):5023-34.

14.

Evidence that 3-phosphoinositide-dependent protein kinase-1 mediates phosphorylation of p70 S6 kinase in vivo at Thr-412 as well as Thr-252.

Balendran A, Currie R, Armstrong CG, Avruch J, Alessi DR.

J Biol Chem. 1999 Dec 24;274(52):37400-6.

15.

An evaluation of fluorescence polarization and lifetime discriminated polarization for high throughput screening of serine/threonine kinases.

Fowler A, Swift D, Longman E, Acornley A, Hemsley P, Murray D, Unitt J, Dale I, Sullivan E, Coldwell M.

Anal Biochem. 2002 Sep 15;308(2):223-31.

PMID:
12419333
16.

PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2.

Balendran A, Casamayor A, Deak M, Paterson A, Gaffney P, Currie R, Downes CP, Alessi DR.

Curr Biol. 1999 Apr 22;9(8):393-404.

17.

The role of 3-phosphoinositide-dependent protein kinase 1 in activating AGC kinases defined in embryonic stem cells.

Williams MR, Arthur JS, Balendran A, van der Kaay J, Poli V, Cohen P, Alessi DR.

Curr Biol. 2000 Apr 20;10(8):439-48.

18.

A high-throughput assay based on fluorescence polarization for inhibitors of the polo-box domain of polo-like kinase 1.

Reindl W, Strebhardt K, Berg T.

Anal Biochem. 2008 Dec 15;383(2):205-9. doi: 10.1016/j.ab.2008.08.014.

PMID:
18793607
19.

Inhibition of Akt kinase activity by a peptide spanning the betaA strand of the proto-oncogene TCL1.

Hiromura M, Okada F, Obata T, Auguin D, Shibata T, Roumestand C, Noguchi M.

J Biol Chem. 2004 Dec 17;279(51):53407-18.

20.

A homogeneous fluorescence polarization assay adaptable for a range of protein serine/threonine and tyrosine kinases.

Gaudet EA, Huang KS, Zhang Y, Huang W, Mark D, Sportsman JR.

J Biomol Screen. 2003 Apr;8(2):164-75.

PMID:
12844437
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