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Items: 1 to 20 of 106

1.

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

Kawarasaki Y, Griswold KE, Stevenson JD, Selzer T, Benkovic SJ, Iverson BL, Georgiou G.

Nucleic Acids Res. 2003 Nov 1;31(21):e126.

2.

Evolution of highly active enzymes by homology-independent recombination.

Griswold KE, Kawarasaki Y, Ghoneim N, Benkovic SJ, Iverson BL, Georgiou G.

Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10082-7.

3.

Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification.

Ikeuchi A, Kawarasaki Y, Shinbata T, Yamane T.

Biotechnol Prog. 2003 Sep-Oct;19(5):1460-7.

PMID:
14524707
4.

Creating multiple-crossover DNA libraries independent of sequence identity.

Lutz S, Ostermeier M, Moore GL, Maranas CD, Benkovic SJ.

Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11248-53.

5.

A combinatorial approach to hybrid enzymes independent of DNA homology.

Ostermeier M, Shim JH, Benkovic SJ.

Nat Biotechnol. 1999 Dec;17(12):1205-9.

PMID:
10585719
6.

The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase.

Griswold KE, Aiyappan NS, Iverson BL, Georgiou G.

J Mol Biol. 2006 Dec 1;364(3):400-10.

8.

Structural determinants of glutathione transferases with azathioprine activity identified by DNA shuffling of alpha class members.

Kurtovic S, Modén O, Shokeer A, Mannervik B.

J Mol Biol. 2008 Feb 1;375(5):1365-79.

PMID:
18155239
9.

Automating gene library synthesis by structure-based combinatorial protein engineering: examples from plant sesquiterpene synthases.

Dokarry M, Laurendon C, O'Maille PE.

Methods Enzymol. 2012;515:21-42. doi: 10.1016/B978-0-12-394290-6.00002-1.

PMID:
22999168
10.

Incremental truncation as a strategy in the engineering of novel biocatalysts.

Ostermeier M, Nixon AE, Benkovic SJ.

Bioorg Med Chem. 1999 Oct;7(10):2139-44.

PMID:
10579518
11.

Use of chimeras generated by DNA shuffling: probing structure-function relationships among glutathione transferases.

Hansson LO, Mannervik B.

Methods Enzymol. 2000;328:463-77. No abstract available.

PMID:
11075361
12.

Sequence-independent construction of ordered combinatorial libraries with predefined crossover points.

Jézéquel L, Loeper J, Pompon D.

Biotechniques. 2008 Nov;45(5):523-32. doi: 10.2144/000112954.

13.

Evolution of differential substrate specificities in Mu class glutathione transferases probed by DNA shuffling.

Hansson LO, Bolton-Grob R, Massoud T, Mannervik B.

J Mol Biol. 1999 Mar 26;287(2):265-76.

PMID:
10080890
14.

Analysis of shuffled gene libraries.

Joern JM, Meinhold P, Arnold FH.

J Mol Biol. 2002 Feb 22;316(3):643-56.

PMID:
11866523
16.

DNA shuffling method for generating highly recombined genes and evolved enzymes.

Coco WM, Levinson WE, Crist MJ, Hektor HJ, Darzins A, Pienkos PT, Squires CH, Monticello DJ.

Nat Biotechnol. 2001 Apr;19(4):354-9.

PMID:
11283594
17.

Combinatorial recombination of gene fragments to construct a library of chimeras.

Farrow MF, Arnold FH.

Curr Protoc Protein Sci. 2010 Aug;Chapter 26:Unit 26.2. doi: 10.1002/0471140864.ps2602s61.

PMID:
20814931
18.

USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction.

Villiers BR, Stein V, Hollfelder F.

Protein Eng Des Sel. 2010 Jan;23(1):1-8. doi: 10.1093/protein/gzp063.

PMID:
19897542
19.

Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity.

van der Veen BA, Potocki-Véronèse G, Albenne C, Joucla G, Monsan P, Remaud-Simeon M.

FEBS Lett. 2004 Feb 27;560(1-3):91-7.

20.

Polishing the craft of genetic diversity creation in directed evolution.

Tee KL, Wong TS.

Biotechnol Adv. 2013 Dec;31(8):1707-21. doi: 10.1016/j.biotechadv.2013.08.021. Review.

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