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Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae.

Flikweert MT, de Swaaf M, van Dijken JP, Pronk JT.

FEMS Microbiol Lett. 1999 May 1;174(1):73-9.


Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast.

van Maris AJ, Geertman JM, Vermeulen A, Groothuizen MK, Winkler AA, Piper MD, van Dijken JP, Pronk JT.

Appl Environ Microbiol. 2004 Jan;70(1):159-66.


Pyruvate decarboxylase: an indispensable enzyme for growth of Saccharomyces cerevisiae on glucose.

Flikweert MT, Van Der Zanden L, Janssen WM, Steensma HY, Van Dijken JP, Pronk JT.

Yeast. 1996 Mar 15;12(3):247-57.


Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain.

Zhang Y, Liu G, Engqvist MK, Krivoruchko A, Hallström BM, Chen Y, Siewers V, Nielsen J.

Microb Cell Fact. 2015 Aug 8;14:116. doi: 10.1186/s12934-015-0305-6.


An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae.

Oud B, Flores CL, Gancedo C, Zhang X, Trueheart J, Daran JM, Pronk JT, van Maris AJ.

Microb Cell Fact. 2012 Sep 15;11:131. doi: 10.1186/1475-2859-11-131.


Steady-state and transient-state analysis of growth and metabolite production in a Saccharomyces cerevisiae strain with reduced pyruvate-decarboxylase activity.

Flikweert MT, Kuyper M, van Maris AJ, Kötter P, van Dijken JP, Pronk JT.

Biotechnol Bioeng. 1999;66(1):42-50.


Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae.

Postma E, Verduyn C, Scheffers WA, Van Dijken JP.

Appl Environ Microbiol. 1989 Feb;55(2):468-77.


Metabolic responses of pyruvate decarboxylase-negative Saccharomyces cerevisiae to glucose excess.

Flikweert MT, van Dijken JP, Pronk JT.

Appl Environ Microbiol. 1997 Sep;63(9):3399-404.


Effects of pyruvate decarboxylase overproduction on flux distribution at the pyruvate branch point in Saccharomyces cerevisiae.

van Hoek P, Flikweert MT, van der Aart QJ, Steensma HY, van Dijken JP, Pronk JT.

Appl Environ Microbiol. 1998 Jun;64(6):2133-40.


Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis.

Monschau N, Stahmann KP, Sahm H, McNeil JB, Bognar AL.

FEMS Microbiol Lett. 1997 May 1;150(1):55-60.


Engineering acetyl coenzyme A supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of Saccharomyces cerevisiae.

Kozak BU, van Rossum HM, Luttik MA, Akeroyd M, Benjamin KR, Wu L, de Vries S, Daran JM, Pronk JT, van Maris AJ.

MBio. 2014 Oct 21;5(5):e01696-14. doi: 10.1128/mBio.01696-14.


Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases by alternative pathways for cytosolic acetyl-CoA synthesis.

Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van Maris AJ.

Metab Eng. 2014 Jan;21:46-59. doi: 10.1016/j.ymben.2013.11.005. Epub 2013 Nov 19.


Ach1 is involved in shuttling mitochondrial acetyl units for cytosolic C2 provision in Saccharomyces cerevisiae lacking pyruvate decarboxylase.

Chen Y, Zhang Y, Siewers V, Nielsen J.

FEMS Yeast Res. 2015 May;15(3). pii: fov015. doi: 10.1093/femsyr/fov015. Epub 2015 Apr 6.


Energetic aspects of glucose metabolism in a pyruvate-dehydrogenase-negative mutant of Saccharomyces cerevisiae.

Pronk JT, Wenzel TJ, Luttik MA, Klaassen CC, Scheffers WA, Steensma HY, van Dijken JP.

Microbiology. 1994 Mar;140 ( Pt 3):601-10.


Regulation of pyruvate metabolism in chemostat cultures of Kluyveromyces lactis CBS 2359.

Zeeman AM, Kuyper M, Pronk JT, van Dijken JP, Steensma HY.

Yeast. 2000 May;16(7):611-20.


Anaplerotic role for cytosolic malic enzyme in engineered Saccharomyces cerevisiae strains.

Zelle RM, Harrison JC, Pronk JT, van Maris AJ.

Appl Environ Microbiol. 2011 Feb;77(3):732-8. doi: 10.1128/AEM.02132-10. Epub 2010 Dec 3.


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