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Items: 1 to 20 of 80

1.

Mechanistic and kinetic considerations of protein splicing.

Evans TC JR TC, Xu MQ.

Chem Rev. 2002 Dec;102(12):4869-84. Review. No abstract available.

PMID:
12475209
2.

"The splice is right": how protein splicing is opening new doors in protein science.

Schwartz EC, Muir TW, Tyszkiewicz AB.

Chem Commun (Camb). 2003 Sep 7;(17):2087-90. Review. No abstract available.

PMID:
13678154
3.

Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions.

Sharma S, Zhang A, Wang H, Harcum SW, Chong S.

Biotechnol Prog. 2003 May-Jun;19(3):1085-90.

PMID:
12790686
4.

Sortase-mediated ligation: a gift from Gram-positive bacteria to protein engineering.

Tsukiji S, Nagamune T.

Chembiochem. 2009 Mar 23;10(5):787-98. doi: 10.1002/cbic.200800724. Review. No abstract available.

PMID:
19199328
5.

Fine-tuning an engineered intein.

Amitai G, Pietrokovski S.

Nat Biotechnol. 1999 Sep;17(9):854-5. No abstract available.

PMID:
10471922
6.

Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing.

Ludwig C, Pfeiff M, Linne U, Mootz HD.

Angew Chem Int Ed Engl. 2006 Aug 4;45(31):5218-21. No abstract available.

PMID:
16823791
7.

Improved protein splicing reaction for low solubility protein fragments without insertion of native extein residues.

Kamioka T, Tawa M, Sohya S, Yamazaki T, Kuroda Y.

Biopolymers. 2009;92(5):465-70. doi: 10.1002/bip.21261.

PMID:
19462417
8.

[Utilizing of protein splicing for human growth hormone purification].

Starokadomskiĭ PL, Okunev OV, Irodov DM, Kordium VA.

Mol Biol (Mosk). 2008 Nov-Dec;42(6):1085-92. Russian.

PMID:
19140330
9.
10.

Novel split intein for trans-splicing synthetic peptide onto C terminus of protein.

Appleby JH, Zhou K, Volkmann G, Liu XQ.

J Biol Chem. 2009 Mar 6;284(10):6194-9. doi: 10.1074/jbc.M805474200. Epub 2009 Jan 9.

11.

Protein ligation: an enabling technology for the biophysical analysis of proteins.

Muralidharan V, Muir TW.

Nat Methods. 2006 Jun;3(6):429-38. Review.

PMID:
16721376
12.

Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression.

Nichols NM, Benner JS, Martin DD, Evans TC Jr.

Biochemistry. 2003 May 13;42(18):5301-11.

PMID:
12731871
13.

[Preparation of mutant enzymes by means of refolding of polypeptide forming inclusion bodies and secretory expression system].

Nishiyama M, Beppu T.

Tanpakushitsu Kakusan Koso. 1994 May;39(7):1366-72. Review. Japanese. No abstract available.

PMID:
8016370
14.

Protein engineering towards biotechnological production of bifunctional polyester beads.

Atwood JA, Rehm BH.

Biotechnol Lett. 2009 Jan;31(1):131-7. doi: 10.1007/s10529-008-9836-9. Epub 2008 Sep 18.

PMID:
18800192
15.

Semisynthesis of proteins using split inteins.

Ludwig C, Schwarzer D, Zettler J, Garbe D, Janning P, Czeslik C, Mootz HD.

Methods Enzymol. 2009;462:77-96. doi: 10.1016/S0076-6879(09)62004-8.

PMID:
19632470
16.

A novel method for increasing the expression level of recombinant proteins.

Wang A, Clapper J, Guderian JA, Foy TM, Fanger GR, Retter MW, Skeiky YA.

Protein Expr Purif. 2003 Jul;30(1):124-33.

PMID:
12821330
17.

Purification of recombinant proteins from E. coli by engineered inteins.

Xu MQ, Evans TC Jr.

Methods Mol Biol. 2003;205:43-68. No abstract available.

PMID:
12491879
18.

A thermostable enzyme as an experimental platform to study properties of less stable homologues.

Lill H, Hisabori T, Groth G, Bald D.

Protein Eng Des Sel. 2004 Jul;17(7):553-5. Epub 2004 Aug 27.

PMID:
15333775
19.

Molecular cloning and expression in yeast of caprine prochymosin.

Vega-Hernández MC, Gómez-Coello A, Villar J, Claverie-Martín F.

J Biotechnol. 2004 Oct 19;114(1-2):69-79.

PMID:
15464600
20.

[Intein-containing chimeric protein construction and evaluation of its cleavage conditions].

Starokadomskiĭ PL, Dubeĭ IIa, Okunev OV, Irodov DM.

Tsitol Genet. 2007 Mar-Apr;41(2):3-11. Russian.

PMID:
17494337

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