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Items: 1 to 20 of 113

1.

Single-cell transcriptome conservation in cryopreserved cells and tissues.

Guillaumet-Adkins A, Rodríguez-Esteban G, Mereu E, Mendez-Lago M, Jaitin DA, Villanueva A, Vidal A, Martinez-Marti A, Felip E, Vivancos A, Keren-Shaul H, Heath S, Gut M, Amit I, Gut I, Heyn H.

Genome Biol. 2017 Mar 1;18(1):45. doi: 10.1186/s13059-017-1171-9.

2.

Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons.

Krishnaswami SR, Grindberg RV, Novotny M, Venepally P, Lacar B, Bhutani K, Linker SB, Pham S, Erwin JA, Miller JA, Hodge R, McCarthy JK, Kelder M, McCorrison J, Aevermann BD, Fuertes FD, Scheuermann RH, Lee J, Lein ES, Schork N, McConnell MJ, Gage FH, Lasken RS.

Nat Protoc. 2016 Mar;11(3):499-524. doi: 10.1038/nprot.2016.015. Epub 2016 Feb 18.

3.

Comparative Analysis of Single-Cell RNA Sequencing Methods.

Ziegenhain C, Vieth B, Parekh S, Reinius B, Guillaumet-Adkins A, Smets M, Leonhardt H, Heyn H, Hellmann I, Enard W.

Mol Cell. 2017 Feb 16;65(4):631-643.e4. doi: 10.1016/j.molcel.2017.01.023.

4.

Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq.

Macaulay IC, Teng MJ, Haerty W, Kumar P, Ponting CP, Voet T.

Nat Protoc. 2016 Nov;11(11):2081-103. doi: 10.1038/nprot.2016.138. Epub 2016 Sep 29.

PMID:
27685099
5.

High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis.

Wang W, Penland L, Gokce O, Croote D, Quake SR.

BMC Genomics. 2018 Feb 13;19(1):140. doi: 10.1186/s12864-018-4512-5.

6.

Single-cell transcriptome analysis of endometrial tissue.

Krjutškov K, Katayama S, Saare M, Vera-Rodriguez M, Lubenets D, Samuel K, Laisk-Podar T, Teder H, Einarsdottir E, Salumets A, Kere J.

Hum Reprod. 2016 Apr;31(4):844-53. doi: 10.1093/humrep/dew008. Epub 2016 Feb 13.

7.

RNA-stabilized whole blood samples but not peripheral blood mononuclear cells can be stored for prolonged time periods prior to transcriptome analysis.

Debey-Pascher S, Hofmann A, Kreusch F, Schuler G, Schuler-Thurner B, Schultze JL, Staratschek-Jox A.

J Mol Diagn. 2011 Jul;13(4):452-60. doi: 10.1016/j.jmoldx.2011.03.006.

8.

Profiling of Single-Cell Transcriptomes.

Chen W, Gardeux V, Meireles-Filho A, Deplancke B.

Curr Protoc Mouse Biol. 2017 Sep 8;7(3):145-175. doi: 10.1002/cpmo.30.

PMID:
28884792
9.

RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.

Pellegrino M, Sciambi A, Yates JL, Mast JD, Silver C, Eastburn DJ.

BMC Genomics. 2016 May 17;17:361. doi: 10.1186/s12864-016-2694-2.

10.

Full-length RNA-seq from single cells using Smart-seq2.

Picelli S, Faridani OR, Björklund AK, Winberg G, Sagasser S, Sandberg R.

Nat Protoc. 2014 Jan;9(1):171-81. doi: 10.1038/nprot.2014.006. Epub 2014 Jan 2.

PMID:
24385147
11.

CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq.

Hashimshony T, Senderovich N, Avital G, Klochendler A, de Leeuw Y, Anavy L, Gennert D, Li S, Livak KJ, Rozenblatt-Rosen O, Dor Y, Regev A, Yanai I.

Genome Biol. 2016 Apr 28;17:77. doi: 10.1186/s13059-016-0938-8.

12.

A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers.

Rosales SL, Liang S, Engel I, Schmiedel BJ, Kronenberg M, Vijayanand P, Seumois G.

Methods Mol Biol. 2018;1799:275-301. doi: 10.1007/978-1-4939-7896-0_21.

PMID:
29956159
13.

Single-cell RNA-sequencing: The future of genome biology is now.

Picelli S.

RNA Biol. 2017 May 4;14(5):637-650. doi: 10.1080/15476286.2016.1201618. Epub 2016 Jul 21. Review.

14.

A platform independent RNA-Seq protocol for the detection of transcriptome complexity.

Calabrese C, Mangiulli M, Manzari C, Paluscio AM, Caratozzolo MF, Marzano F, Kurelac I, D'Erchia AM, D'Elia D, Licciulli F, Liuni S, Picardi E, Attimonelli M, Gasparre G, Porcelli AM, Pesole G, Sbisà E, Tullo A.

BMC Genomics. 2013 Dec 5;14:855. doi: 10.1186/1471-2164-14-855.

15.

Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

Hedegaard J, Thorsen K, Lund MK, Hein AM, Hamilton-Dutoit SJ, Vang S, Nordentoft I, Birkenkamp-Demtröder K, Kruhøffer M, Hager H, Knudsen B, Andersen CL, Sørensen KD, Pedersen JS, Ørntoft TF, Dyrskjøt L.

PLoS One. 2014 May 30;9(5):e98187. doi: 10.1371/journal.pone.0098187. eCollection 2014.

16.

Smart-seq2 for sensitive full-length transcriptome profiling in single cells.

Picelli S, Björklund ÅK, Faridani OR, Sagasser S, Winberg G, Sandberg R.

Nat Methods. 2013 Nov;10(11):1096-8. doi: 10.1038/nmeth.2639. Epub 2013 Sep 22.

PMID:
24056875
17.

Quantitative assessment of single-cell RNA-sequencing methods.

Wu AR, Neff NF, Kalisky T, Dalerba P, Treutlein B, Rothenberg ME, Mburu FM, Mantalas GL, Sim S, Clarke MF, Quake SR.

Nat Methods. 2014 Jan;11(1):41-6. doi: 10.1038/nmeth.2694. Epub 2013 Oct 20.

18.

Mining the Archives: A Cross-Platform Analysis of Gene Expression Profiles in Archival Formalin-Fixed Paraffin-Embedded Tissues.

Webster AF, Zumbo P, Fostel J, Gandara J, Hester SD, Recio L, Williams A, Wood CE, Yauk CL, Mason CE.

Toxicol Sci. 2015 Dec;148(2):460-72. doi: 10.1093/toxsci/kfv195. Epub 2015 Sep 10.

19.

Are cells from a snowman realistic? Cryopreserved tissues as a source for single-cell RNA-sequencing experiments.

Vieira Braga FA, Teichmann SA, Stubbington MJ.

Genome Biol. 2017 Mar 24;18(1):54. doi: 10.1186/s13059-017-1192-4. Review.

20.

Transcriptomics in the RNA-seq era.

McGettigan PA.

Curr Opin Chem Biol. 2013 Feb;17(1):4-11. doi: 10.1016/j.cbpa.2012.12.008. Epub 2013 Jan 2. Review.

PMID:
23290152

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