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Items: 1 to 20 of 138

1.

Detection and Quantification of Intracellular Signaling Using FRET-Based Biosensors and High Content Imaging.

Halls ML, Poole DP, Ellisdon AM, Nowell CJ, Canals M.

Methods Mol Biol. 2015;1335:131-61. doi: 10.1007/978-1-4939-2914-6_10.

PMID:
26260599
2.

FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells.

Kraft AE, Nikolaev VO.

Methods Mol Biol. 2017;1563:85-90. doi: 10.1007/978-1-4939-6810-7_6.

PMID:
28324603
3.
4.

Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons.

Oliveira AF, Yasuda R.

Methods Mol Biol. 2014;1071:109-28. doi: 10.1007/978-1-62703-622-1_9.

PMID:
24052384
5.

Stable expression of FRET biosensors: a new light in cancer research.

Aoki K, Komatsu N, Hirata E, Kamioka Y, Matsuda M.

Cancer Sci. 2012 Apr;103(4):614-9. doi: 10.1111/j.1349-7006.2011.02196.x. Epub 2012 Feb 3. Review.

6.

Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors.

Gesellchen F, Stangherlin A, Surdo N, Terrin A, Zoccarato A, Zaccolo M.

Methods Mol Biol. 2011;746:297-316. doi: 10.1007/978-1-61779-126-0_16.

PMID:
21607864
7.

Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: basis of biosensor construction, live imaging, and image processing.

Aoki K, Kamioka Y, Matsuda M.

Dev Growth Differ. 2013 May;55(4):515-22. doi: 10.1111/dgd.12039. Epub 2013 Feb 7. Review.

PMID:
23387795
8.

Genetically encoded FRET-based biosensors for multiparameter fluorescence imaging.

Carlson HJ, Campbell RE.

Curr Opin Biotechnol. 2009 Feb;20(1):19-27. doi: 10.1016/j.copbio.2009.01.003. Epub 2009 Feb 14.

PMID:
19223167
9.

FRET microscopy for real-time monitoring of signaling events in live cells using unimolecular biosensors.

Sprenger JU, Perera RK, Götz KR, Nikolaev VO.

J Vis Exp. 2012 Aug 20;(66):e4081. doi: 10.3791/4081.

10.

A versatile toolkit to produce sensitive FRET biosensors to visualize signaling in time and space.

Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O.

Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.

PMID:
23882122
11.

Quantitative measurement of cAMP concentration using an exchange protein directly activated by a cAMP-based FRET-sensor.

Salonikidis PS, Zeug A, Kobe F, Ponimaskin E, Richter DW.

Biophys J. 2008 Dec;95(11):5412-23. doi: 10.1529/biophysj.107.125666. Epub 2008 Aug 15.

12.

An introduction to fluorescence imaging techniques geared towards biosensor applications.

Goedhart J, Hink MA, Jalink K.

Methods Mol Biol. 2014;1071:17-28. doi: 10.1007/978-1-62703-622-1_2. Review.

PMID:
24052377
13.

Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors.

Aoki K, Matsuda M.

Nat Protoc. 2009;4(11):1623-31. doi: 10.1038/nprot.2009.175. Epub 2009 Oct 15.

PMID:
19834477
14.

Optimization of ERK activity biosensors for both ratiometric and lifetime FRET measurements.

Vandame P, Spriet C, Riquet F, Trinel D, Cailliau-Maggio K, Bodart JF.

Sensors (Basel). 2014 Jan 10;14(1):1140-54. doi: 10.3390/s140101140.

15.

Dynamic visualization of cellular signaling.

Ni Q, Zhang J.

Adv Biochem Eng Biotechnol. 2010;119:79-97. doi: 10.1007/10_2008_48. Review.

PMID:
19499207
16.

Cellular Application of Genetically Encoded Sensors and Impeders of AMPK.

Miyamoto T, Rho E, Kim A, Inoue T.

Methods Mol Biol. 2018;1732:255-272. doi: 10.1007/978-1-4939-7598-3_17.

PMID:
29480481
17.

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.

Ai HW, Hazelwood KL, Davidson MW, Campbell RE.

Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207. Epub 2008 Apr 20.

PMID:
18425137
18.

Recording intracellular cAMP levels with EPAC-based FRET sensors by fluorescence lifetime imaging.

Raspe M, Klarenbeek J, Jalink K.

Methods Mol Biol. 2015;1294:13-24. doi: 10.1007/978-1-4939-2537-7_2.

PMID:
25783874
19.

Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM.

Chennell G, Willows RJ, Warren SC, Carling D, French PM, Dunsby C, Sardini A.

Sensors (Basel). 2016 Aug 19;16(8). pii: E1312. doi: 10.3390/s16081312.

20.

Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM.

Hinde E, Digman MA, Hahn KM, Gratton E.

Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):135-40. doi: 10.1073/pnas.1211882110. Epub 2012 Dec 17.

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