Spatial analysis of spike-timing-dependent LTP and LTD in the CA1 area of hippocampal slices using optical imaging

Minoru Tsukada; Takeshi Aihara; Yuki Kobayashi; Hideaki Shimazaki

doi:10.1002/hipo.20035

Spatial analysis of spike-timing-dependent LTP and LTD in the CA1 area of hippocampal slices using optical imaging

Hippocampus. 2005;15(1):104-9. doi: 10.1002/hipo.20035.

Authors

Minoru Tsukada¹, Takeshi Aihara, Yuki Kobayashi, Hideaki Shimazaki

Affiliation

¹ Department of Information and Communication Engineering, Faculty of Engineering, Tamagawa University, Tokyo, Japan. tsukada@eng.tamagawa.ac.jp

PMID: 15390160
DOI: 10.1002/hipo.20035

Abstract

Spike-timing-dependent long-term potentiation (LTP) and long-term depression (LTD) were investigated in the CA1 area of hippocampal slices using optical imaging. A pair of electrical pulses were used to stimulate the Schaffer-commissural collateral and the stratum oriens with various sets of relative timing (tau) between the two stimuli. These sets of paired pulses gave rise to LTP/LTD, whose induction was closely related to tau, and their profiles were classified into two types depending on their layer specific location along the dendrite. One was characterized by a symmetric time window observed in the proximal region of the stratum radiatum (SR) and the other by an asymmetric time window in the distal region of the SR. Bath application of bicuculline (gamma-aminobutyric acid [GABA] receptor antagonist) to hippocampal slices revealed that GABAergic interneuron projections were responsible for the symmetry of a time window.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Action Potentials / drug effects
Action Potentials / physiology*
Animals
Dendrites / drug effects
Dendrites / physiology
Electric Stimulation
Electrophysiology / methods
Female
GABA Antagonists / pharmacology
Hippocampus / anatomy & histology
Hippocampus / drug effects
Hippocampus / physiology*
Interneurons / drug effects
Interneurons / metabolism
Long-Term Potentiation / drug effects
Long-Term Potentiation / physiology*
Long-Term Synaptic Depression / drug effects
Long-Term Synaptic Depression / physiology*
Neural Inhibition / drug effects
Neural Inhibition / physiology
Neural Pathways / anatomy & histology
Neural Pathways / drug effects
Neural Pathways / physiology*
Neurons / physiology*
Neurophysiology / methods
Optics and Photonics / instrumentation
Organ Culture Techniques
Rats
Rats, Wistar
Reaction Time / drug effects
Reaction Time / physiology
Synaptic Transmission / drug effects
Synaptic Transmission / physiology
Time Factors
gamma-Aminobutyric Acid / metabolism

Substances

GABA Antagonists
gamma-Aminobutyric Acid