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Curr Protoc Microbiol. 2016 Nov 18;43:14B.9.1-14B.9.20. doi: 10.1002/cpmc.16.

Detection and Genotyping of Human Papillomaviruses from Archival Formalin-Fixed Tissue Samples.

Author information

1
Lab of Viral Diseases, NIAID, NIH, Bethesda, Maryland.
2
Department of Microbiology, The Chinese University of Hong Kong, Hong Kong.

Abstract

Pathology departments routinely process and store formalin-fixed, paraffin-embedded (FFPE) tissue samples for clinical diagnosis. These collections often contain decades' worth of samples and represent a treasure trove of specimens that can be analyzed for retrospective epidemiological studies, diagnostics, and pathogen discovery. Accurate amplification and sequencing of DNA from these samples is critical for the usability of these FFPE samples. Here we present a collection of protocols that describe extraction of DNA from FFPE tissues, PCR amplification of human papillomavirus DNA, and subsequent genotyping of the infecting virus.

KEYWORDS:

FFPE; HPV; PCR; cancer; cervix; degenerate primers; formalin; neoplasia; papillomavirus; sequencing

PMID:
27858973
PMCID:
PMC5117989
DOI:
10.1002/cpmc.16
[Indexed for MEDLINE]
Free PMC Article

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