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North Clin Istanb. 2018 Aug 8;6(4):348-354. doi: 10.14744/nci.2018.37132. eCollection 2019.

Evaluation of serum thiol/disulfide homeostasis in patients with ankylosing spondylitis by a novel method.

Author information

1
Department of Physical Medicine and Rehabilitation, Malatya Training and Research Hospital, Malatya, Turkey.
2
Department of Biochemistry, Malatya Training and Research Hospital, Malatya, Turkey.
3
Division of Rheumatology, Department of Internal Medicine, Numune Training and Research Hospital, Ankara, Turkey.
4
Department of Physical Medicine and Rehabilitation, Private Malatya Park Hospital, Malatya, Turkey.

Abstract

OBJECTIVE:

Increased reactive oxygen species may play an important role in Ankylosing spondylitis (AS) etiopathogenesis. The thiol group is a very potent antioxidant. In this study, we aimed to investigate the presence of oxidative stress in patients with AS by evaluating thiol/disulfide homeostasis.

METHODS:

In this study, a total of 66 AS patients (27 male, 39 female) and 66 healthy controls (21 male, 45 female) were enrolled. Recently, a novel method for the thiol measurement was found. Thiol and disulfide values were measured by the novel methods.

RESULTS:

Native thiol (NT) (p<0.001) and native thiol/total thiol (NTT) (p<0.001) levels of AS patients were significantly lower compared to the values of the healthy group. However, disulfide (p<0.001), disulfide/native thiol (DNT) (p<0.001) and disulfide/total thiol (DTT) levels of AS patients were a strongly higher control group. A negative correlation was found between BASFI and NTT. Also, a negative correlation was found between BASDAI and NT, NTT levels. A positive correlation was found between BASFI and disulfide, DNT and DTT levels. A positive correlation was found between BASDAI and disulfide, DNT and DTT levels.

CONCLUSION:

The findings revealed that thiol-disulfide homeostasis deteriorated in patients with AS in favor of disulfide amounts. Thiol-disulfide homeostasis can play roles in the etiology and severity of AS.

KEYWORDS:

Ankylosing spondylitis; disulfide; oxidative stress; thiol; thiol/disulfide homeostasis

Conflict of interest statement

Conflict of Interest: The authors declare no conflict of interest.

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