Format

Send to

Choose Destination
See comment in PubMed Commons below
Retrovirology. 2005 Sep 29;2:60.

Dicistronic MLV-retroviral vectors transduce neural precursors in vivo and co-express two genes in their differentiated neuronal progeny.

Author information

1
LaboRétro, INSERM U412, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, Lyon 69364 Cedex 07, France. derrington@cgmc.univ-lyon1.fr

Abstract

Dicistronic MLV-based retroviral vectors, in which two IRESes independently initiate the translation of two proteins from a single RNA, have been shown to direct co-expression of proteins in several cell culture systems. Here we report that these dicistronic retroviral vectors can drive co-expression of two gene products in brain cells in vivo. Injection of retroviral vector producer cells leads to the transduction of proliferating precursors in the external granular layer of the cerebellum and throughout the ventricular regions. Differentiated neurons co-expressing both transgenes were observed in the cerebellum and in lower numbers in distant brain regions such as the cortex. Thus, we describe an eukaryotic dicistronic vector system that is capable of transducing mouse neural precursors in vivo and maintaining the expression of genes after cell differentiation.

PMID:
16194277
PMCID:
PMC1266060
DOI:
10.1186/1742-4690-2-60
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Support Center