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Items: 1 to 20 of 84

1.

Determination of the catalytic base in family 48 glycosyl hydrolases.

Kostylev M, Wilson DB.

Appl Environ Microbiol. 2011 Sep;77(17):6274-6. doi: 10.1128/AEM.05532-11. Epub 2011 Jul 15.

2.
3.

The absence of an identifiable single catalytic base residue in Thermobifida fusca exocellulase Cel6B.

Vuong TV, Wilson DB.

FEBS J. 2009 Jul;276(14):3837-45. doi: 10.1111/j.1742-4658.2009.07097.x. Epub 2009 Jun 11.

4.
5.

Site-directed mutagenesis to probe catalysis by a Thermobifida fusca beta-1,3-glucanase (Lam81A).

McGrath CE, Vuong TV, Wilson DB.

Protein Eng Des Sel. 2009 Jun;22(6):375-82. doi: 10.1093/protein/gzp015. Epub 2009 May 12.

PMID:
19435780
6.

Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies.

Vallmitjana M, Ferrer-Navarro M, Planell R, Abel M, Ausín C, Querol E, Planas A, Pérez-Pons JA.

Biochemistry. 2001 May 22;40(20):5975-82.

PMID:
11352732
7.

The identification of the acid-base catalyst of alpha-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase.

Shallom D, Belakhov V, Solomon D, Gilead-Gropper S, Baasov T, Shoham G, Shoham Y.

FEBS Lett. 2002 Mar 13;514(2-3):163-7.

8.

Site-directed mutagenesis of α-L-rhamnosidase from Alternaria sp. L1 to enhance synthesis yield of reverse hydrolysis based on rational design.

Xu L, Liu X, Yin Z, Liu Q, Lu L, Xiao M.

Appl Microbiol Biotechnol. 2016 Dec;100(24):10385-10394. Epub 2016 Jun 28.

PMID:
27352363
9.

Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family 97.

Okuyama M, Kitamura M, Hondoh H, Kang MS, Mori H, Kimura A, Tanaka I, Yao M.

J Mol Biol. 2009 Oct 9;392(5):1232-41. doi: 10.1016/j.jmb.2009.07.068. Epub 2009 Jul 30.

PMID:
19646996
10.

Probing the catalytically essential residues of the alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus.

Debeche T, Bliard C, Debeire P, O'Donohue MJ.

Protein Eng. 2002 Jan;15(1):21-8.

PMID:
11842234
11.

A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains.

Cho KM, Hong SY, Lee SM, Kim YH, Kahng GG, Kim H, Yun HD.

Appl Microbiol Biotechnol. 2006 Dec;73(3):618-30. Epub 2006 Aug 16.

PMID:
16912849
12.

Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase.

Joshi MD, Sidhu G, Pot I, Brayer GD, Withers SG, McIntosh LP.

J Mol Biol. 2000 May 26;299(1):255-79.

PMID:
10860737
13.

Cel48A from Thermobifida fusca: structure and site directed mutagenesis of key residues.

Kostylev M, Alahuhta M, Chen M, Brunecky R, Himmel ME, Lunin VV, Brady J, Wilson DB.

Biotechnol Bioeng. 2014 Apr;111(4):664-73. doi: 10.1002/bit.25139. Epub 2013 Nov 21.

PMID:
24264519
14.

Detailed kinetic analysis and identification of the nucleophile in alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase.

Shallom D, Belakhov V, Solomon D, Shoham G, Baasov T, Shoham Y.

J Biol Chem. 2002 Nov 15;277(46):43667-73. Epub 2002 Sep 6. Erratum in: J Biol Chem 2002 Dec 27;277(52):51084.

15.

Catalytic mechanism of inulinase from Arthrobacter sp. S37.

Kim KY, Nascimento AS, Golubev AM, Polikarpov I, Kim CS, Kang SI, Kim SI.

Biochem Biophys Res Commun. 2008 Jul 11;371(4):600-5. doi: 10.1016/j.bbrc.2008.03.126. Epub 2008 Apr 3.

PMID:
18395004
16.

Cloning, characterization and phylogenetic relationships of cel5B, a new endoglucanase encoding gene from Thermobifida fusca.

Posta K, Béki E, Wilson DB, Kukolya J, Hornok L.

J Basic Microbiol. 2004;44(5):383-99.

PMID:
15378527
17.
18.

Alternative strategy for converting an inverting glycoside hydrolase into a glycosynthase.

Honda Y, Fushinobu S, Hidaka M, Wakagi T, Shoun H, Taniguchi H, Kitaoka M.

Glycobiology. 2008 Apr;18(4):325-30. doi: 10.1093/glycob/cwn011. Epub 2008 Feb 9.

PMID:
18263897
19.

Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A.

Li Y, Irwin DC, Wilson DB.

Appl Environ Microbiol. 2007 May;73(10):3165-72. Epub 2007 Mar 16.

20.

Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues.

Bolam DN, Hughes N, Virden R, Lakey JH, Hazlewood GP, Henrissat B, Braithwaite KL, Gilbert HJ.

Biochemistry. 1996 Dec 17;35(50):16195-204.

PMID:
8973192

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