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Sangre (Barc). 1995 Feb;40(1):67-9.

[Electrophoresis of 1,149 samples of umbilical cord blood and assessment of the heterogeneity of the relationship between gamma G and gamma A chains of fetal hemoglobin].

[Article in Spanish]

Author information

1
Laboratorio de Genética Humana, I.S.C.M.V.C. La Habana, Cuba.

Abstract

A total number of 1,149 specimens of cord-blood were subjected to cellulose acetate gel electrophoresis in order to estimate the incidence of haemoglobinopathies in newborn infants (NBI). It was found that 4.37% of black NBI and 0.54% of white NBI were AS carriers. Those samples with HbS or HbC were verified by means of agar-citrate electrophoresis at pH 6.2. Haemoglobin Bart was commonest in black (3.1%) than in white (0.4%) NBI. A slow haemoglobin variant was found with an alpha-chain mutation. The concentration of gamma G was estimated by separation of gamma G and gamma A chains with polyacrylamide gel electrophoresis, and it ranged between 52% and 94%, which could be attributed to the fact that the shift from HbF to HbA is not simultaneous in all the red-cell population.

PMID:
7536352
[Indexed for MEDLINE]

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