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Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Feb 9;53(2):85-91. doi: 10.3760/cma.j.issn.1002-0098.2018.02.003.

[Development of a novel absorbable nanofiber chitosan-collagen membrane by electrospinning and the preliminary study on guided bone regeneration].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

Department of Prosthodontics, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.


in English, Chinese

Objective: To evaluate the application effect of nanofiber chitosan-collagen membrane (NCM) on guided bone regeneration (GBR). Methods: The mixture of collagen, chitosan, polyethylene oxide was used to make up the NCM by electrospinning, then the NCM was crosslinked by glutaraldehyde vapor. The physical property of the NCM was measured by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). MC3T3-E1 osteoblasts were cultured on NCM to characterize the biocompatibility. The effectiveness of four groups [contrast group, Bio-gide membrane (BGM), compressed chitosan-collagen menbrane (CCM), NCM/CCM] on bone regeneration were evaluated in critical-sized defects (diameter=5 mm) in SD rats. Results: When the mixed solution consists of 4.0% collagen, 1.0% chitosan and 3.5% polyethylene oxide, the NCM could be validly fabricated by electrospinning. After cross-linking by glutaraldehyde vapor, the tensile strength and the stability of NCM in damp was enhanced. No cytotoxicity of the NCM was detected on MC3T3-E1 osteoblasts. In vivo study showed that the new bone regeneration ratio of NCM/CCM group was [(43.10±1.49)%], and this was similar to that of the group of BGM [(41.36±2.60)%] (P>0.05), but higher than that of the CCM group [(33.10±1.41)%] and the contrast group [(7.22±2.46)%] (P<0.05). Conclusions: The NCM can promote new bone regeneration effectively in GBR procedure.


Bone regeneration; Chitosan; Collagen; Electrospinning

[Indexed for MEDLINE]

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