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Open Dent J. 2017 Aug 31;11:447-459. doi: 10.2174/1874210601711010447. eCollection 2017.

Comparison of Composition and Anticaries Effect of Galla Chinensis Extracts with Different Isolation Methods.

Author information

1
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
2
Department of Cariology, Endodontology, Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
3
Division of General Dentistry, Eastman Institute for Oral Health, University of Rochester, Rochester, NY, USA.
4
Oral Biology Program, School of Dentistry, University of North Carolina, Chapel Hill, NC, USA.
5
Department of Analytical Toxicology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.

Abstract

OBJECTIVES:

Galla chinensis water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods, aiming to improve the efficacy of caries prevention.

METHODS:

The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared. High performance liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) analysis was used to analyze the main ingredients. In vitro pH-cycling regime and polymicrobial biofilms model were used to assess the ability of different Galla chinensis extracts to inhibit enamel demineralization, acid formation and biofilm formation.

RESULTS:

All the GCE, eGCE and tannic acid contained a high level of total phenolics. HPLC-ESI-TOF-MS analysis showed that the main ingredients of GCE were gallic acid (GA), while eGCE mainly contained 4-7 galloylglucopyranoses (GGs) and tannic acid mainly contained 5-10 GGs. Furthermore, eGCE and tannic acid showed a better effect on inhibiting enamel demineralization, acid formation and biofilm formation compared to GCE.

CONCLUSIONS:

Galla chinensis extracts with higher tannin content were suggested to have higher potential to prevent dental caries.

KEYWORDS:

Cariogenic microbiota; Composition; Dental caries; Galla chinensis; Gallic acid

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