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Redox Rep. 2017 Nov;22(6):451-459. doi: 10.1080/13510002.2017.1288973. Epub 2017 Feb 16.

Increased oxidative stress alters nucleosides metabolite levels in sickle cell anemia.

Author information

1
a Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Maria , Santa Maria-RS , Brazil.
2
b Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas , Universidade Federal de Santa Maria , Santa Maria-RS , Brazil.
3
c Department of Biochemistry , Federal University of Technology , Akure , Nigeria.

Abstract

OBJECTIVES:

This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients.

METHODS:

Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCA patients.

RESULTS:

ROS, thiobarbituric acid-reactive substances (TBARS) and T-SH levels as well as the activities of catalase and MPO were significantly increased while glutathione level was reduced in SCA patients. Furthermore, a significant (P < 0.001) increase in hypoxanthine level was demonstrated in SCA patients. However, the serum levels for xanthine (P < 0.01) and uric acid (P < 0.001) were decreased in SCA patients. A significant (P < 0.001) decrease in XO activity was detected in SCA patients.

DISCUSSION:

The altered parameters in SCA patients suggest that the generation and impairment of oxidative stress in this disease as well as antioxidant markers are contributory factors towards cellular redox homeostasis and alteration of purine metabolites.

KEYWORDS:

Sickle cell anemia; enzymes; hypoxanthine; lipid peroxidation; oxidative stress; reactive oxygen species; uric acid; xanthine

PMID:
28209096
DOI:
10.1080/13510002.2017.1288973
[Indexed for MEDLINE]

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