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J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jan 15;1041-1042:167-174. doi: 10.1016/j.jchromb.2016.12.029. Epub 2016 Dec 21.

A method for the preparation of curcumin by ultrasonic-assisted ammonium sulfate/ethanol aqueous two phase extraction.

Author information

1
Laboratory of Biophysics and Biomedical Engineering, School of Physics and Information Technology, Shaanxi Normal University, Xi'an, China.
2
Laboratory of Biophysics and Biomedical Engineering, School of Physics and Information Technology, Shaanxi Normal University, Xi'an, China. Electronic address: Biophymed@snnu.edu.cn.

Abstract

This study investigated a new and easy-to-industrialized extracting method for curcumin from Curcuma longa rhizomes using ultrasonic extraction technology combined with ammonium sulfate/ethanol aqueous two-phase system (ATPS), and the preparation of curcumin using the semi-preparative HPLC. The single-factor experiments and response surface methodology (RSM) were utilized to determine the optimal material-solvent ratio, ultrasonic intensity (UI) and ultrasonic time. The optimum extraction conditions were finally determined to be material-solvent rate of 3.29:100, ultrasonic intensity of 33.63W/cm2 and ultrasonic time of 17min. At these optimum conditions, the extraction yield could reach 46.91mg/g. And the extraction yields of curcumin remained stable in the case of amplification, which indicated that scale-up extraction was feasible and efficient. Afterwards, the semi-preparative HPLC experiment was carried out, in which optimal preparation conditions were elected according to the single factor experiment. The prepared curcumin was obtained and the purity could up to 85.58% by the semi-preparative HPLC.

KEYWORDS:

Aqueous two-phase system; Curcumin; Response surface methodology; Semi-preparative HPLC; Ultrasonic-assisted

PMID:
28043027
DOI:
10.1016/j.jchromb.2016.12.029
[Indexed for MEDLINE]

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