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Arch Virol. 2017 Feb;162(2):543-548. doi: 10.1007/s00705-016-3129-8. Epub 2016 Nov 1.

Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).

Author information

1
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
2
National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland.
3
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
4
Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
5
Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Lusaka, Zambia.
6
Department of Para-clinical studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
7
Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
8
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
9
Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
10
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.
11
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.

Abstract

To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.

PMID:
27804019
DOI:
10.1007/s00705-016-3129-8
[Indexed for MEDLINE]
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