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Appl Environ Microbiol. 2010 Feb;76(3):960-2. doi: 10.1128/AEM.01810-09. Epub 2009 Nov 30.

Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.

Author information

1
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.

Abstract

Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.

PMID:
19948855
PMCID:
PMC2812999
DOI:
10.1128/AEM.01810-09
[Indexed for MEDLINE]
Free PMC Article

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