PMID- 31251760
OWN - NLM
STAT- In-Data-Review
LR  - 20190714
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 6
DP  - 2019
TI  - Development of experimental pneumococcal vaccine for mucosal immunization.
PG  - e0218679
LID - 10.1371/journal.pone.0218679 [doi]
AB  - Streptococcus pneumonia is an important human pathogen that causes various severe
      diseases such as pneumonia, otitis and meningitis. Vaccination against S.
      pneumoniae is implemented in many developed countries. The presently used
      vaccines are safe, well tolerated but relatively expensive and require
      modification due to the immunological changes of the epidemic strains. This paper
      describes the development of a new pneumococcal vaccine candidate for
      immunization on mucosal surfaces. For this purpose the antigens of chimeric
      protein PSPF, previously suggested for an injectable S. pneumoniae vaccine, were 
      expressed on the surface of the live probiotic strain Enterococcus faecium L3.
      Experiments on laboratory mice vaccinated with live bacteria demonstrated the
      appearance of the specific IgA and IgG which provide protection against the
      lethal S. pneumoniae infection.
FAU - Gupalova, Tatiana
AU  - Gupalova T
AD  - Department of Molecular Microbiology, Institute of Experimental Medicine,
      Saint-Petersburg, Russia.
FAU - Leontieva, Galina
AU  - Leontieva G
AUID- ORCID: http://orcid.org/0000-0002-9876-6594
AD  - Department of Molecular Microbiology, Institute of Experimental Medicine,
      Saint-Petersburg, Russia.
FAU - Kramskaya, Tatiana
AU  - Kramskaya T
AD  - Department of Molecular Microbiology, Institute of Experimental Medicine,
      Saint-Petersburg, Russia.
FAU - Grabovskaya, Kornelya
AU  - Grabovskaya K
AD  - Department of Molecular Microbiology, Institute of Experimental Medicine,
      Saint-Petersburg, Russia.
FAU - Kuleshevich, Eugenia
AU  - Kuleshevich E
AD  - Department of Molecular Microbiology, Institute of Experimental Medicine,
      Saint-Petersburg, Russia.
FAU - Suvorov, Alexander
AU  - Suvorov A
AD  - Department of Molecular Microbiology, Institute of Experimental Medicine,
      Saint-Petersburg, Russia.
AD  - Department of Fundamental Medicine and Medical Technologies, Faculty of Dentistry
      and Medical Technologies, Saint Petersburg State University, Saint-Petersburg,
      Russia.
LA  - eng
PT  - Journal Article
DEP - 20190628
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
PMC - PMC6599147
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/06/30 06:00
MHDA- 2019/06/30 06:00
CRDT- 2019/06/29 06:00
PHST- 2019/02/21 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/29 06:00 [entrez]
PHST- 2019/06/30 06:00 [pubmed]
PHST- 2019/06/30 06:00 [medline]
AID - 10.1371/journal.pone.0218679 [doi]
AID - PONE-D-19-05206 [pii]
PST - epublish
SO  - PLoS One. 2019 Jun 28;14(6):e0218679. doi: 10.1371/journal.pone.0218679.
      eCollection 2019.