PMID- 31246981
OWN - NLM
STAT- In-Data-Review
LR  - 20190707
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Linking)
VI  - 13
IP  - 6
DP  - 2019 Jun
TI  - One hypervirulent clone, sequence type 283, accounts for a large proportion of
      invasive Streptococcus agalactiae isolated from humans and diseased tilapia in
      Southeast Asia.
PG  - e0007421
LID - 10.1371/journal.pntd.0007421 [doi]
AB  - BACKGROUND: In 2015, Singapore had the first and only reported foodborne outbreak
      of invasive disease caused by the group B Streptococcus (GBS; Streptococcus
      agalactiae). Disease, predominantly septic arthritis and meningitis, was
      associated with sequence type (ST)283, acquired from eating raw farmed freshwater
      fish. Although GBS sepsis is well-described in neonates and older adults with
      co-morbidities, this outbreak affected non-pregnant and younger adults with fewer
      co-morbidities, suggesting greater virulence. Before 2015 ST283 had only been
      reported from twenty humans in Hong Kong and two in France, and from one fish in 
      Thailand. We hypothesised that ST283 was causing region-wide infection in
      Southeast Asia. METHODOLOGY/PRINCIPAL FINDINGS: We performed a literature review,
      whole genome sequencing on 145 GBS isolates collected from six Southeast Asian
      countries, and phylogenetic analysis on 7,468 GBS sequences including 227
      variants of ST283 from humans and animals. Although almost absent outside Asia,
      ST283 was found in all invasive Asian collections analysed, from 1995 to 2017. It
      accounted for 29/38 (76%) human isolates in Lao PDR, 102/139 (73%) in Thailand,
      4/13 (31%) in Vietnam, and 167/739 (23%) in Singapore. ST283 and its variants
      were found in 62/62 (100%) tilapia from 14 outbreak sites in Malaysia and
      Vietnam, in seven fish species in Singapore markets, and a diseased frog in
      China. CONCLUSIONS: GBS ST283 is widespread in Southeast Asia, where it accounts 
      for a large proportion of bacteraemic GBS, and causes disease and economic loss
      in aquaculture. If human ST283 is fishborne, as in the Singapore outbreak, then
      GBS sepsis in Thailand and Lao PDR is predominantly a foodborne disease. However,
      whether transmission is from aquaculture to humans, or vice versa, or involves an
      unidentified reservoir remains unknown. Creation of cross-border collaborations
      in human and animal health are needed to complete the epidemiological picture.
FAU - Barkham, Timothy
AU  - Barkham T
AUID- ORCID: http://orcid.org/0000-0003-0975-2244
AD  - Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore.
FAU - Zadoks, Ruth N
AU  - Zadoks RN
AD  - Institute of Biodiversity Animal Health and Comparative Medicine, College of
      Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, United
      Kingdom.
FAU - Azmai, Mohammad Noor Amal
AU  - Azmai MNA
AD  - Department of Biology, Faculty of Science, and Laboratory of Marine
      Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Selangor,
      Malaysia.
FAU - Baker, Stephen
AU  - Baker S
AD  - Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
FAU - Bich, Vu Thi Ngoc
AU  - Bich VTN
AD  - Centre for Tropical Medicine, Oxford University Clinical Research Unit, Hanoi,
      Vietnam.
FAU - Chalker, Victoria
AU  - Chalker V
AD  - Public Health England, Colindale, London, United Kingdom.
FAU - Chau, Man Ling
AU  - Chau ML
AD  - Environmental Health Institute, National Environment Agency, Singapore.
AD  - National Centre for Food Science, Singapore Food Agency, Singapore.
FAU - Dance, David
AU  - Dance D
AUID- ORCID: http://orcid.org/0000-0001-9189-7244
AD  - Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital,
      Vientiane, Lao People's Democratic Republic.
AD  - Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, 
      University of Oxford, Oxford, United Kingdom.
AD  - Faculty of Infectious and Tropical Diseases, London School of Hygiene and
      Tropical Medicine, London, United Kingdom.
FAU - Deepak, Rama Narayana
AU  - Deepak RN
AD  - Department of Laboratory Medicine, Khoo Teck Puat Hospital, Singapore.
FAU - van Doorn, H Rogier
AU  - van Doorn HR
AD  - Oxford University Clinical Research Unit, Hanoi, Vietnam.
AD  - Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global
      Health, University of Oxford, United Kingdom.
FAU - Gutierrez, Ramona A
AU  - Gutierrez RA
AD  - Environmental Health Institute, National Environment Agency, Singapore.
AD  - National Centre for Infectious Diseases, Singapore.
FAU - Holmes, Mark A
AU  - Holmes MA
AD  - Department of Veterinary Medicine, University of Cambridge, Cambridge, United
      Kingdom.
FAU - Huong, Lan Nguyen Phu
AU  - Huong LNP
AD  - Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
FAU - Koh, Tse Hsien
AU  - Koh TH
AD  - Department of Microbiology, Singapore General Hospital, Singapore.
FAU - Martins, Elisabete
AU  - Martins E
AUID- ORCID: http://orcid.org/0000-0002-0703-9982
AD  - Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de
      Medicina, Universidade de Lisboa, Lisboa, Portugal.
FAU - Mehershahi, Kurosh
AU  - Mehershahi K
AD  - Department of Medicine, Yong Loo Lin School of Medicine, National University of
      Singapore, Singapore.
FAU - Newton, Paul
AU  - Newton P
AD  - Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital,
      Vientiane, Lao People's Democratic Republic.
AD  - Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, 
      University of Oxford, Oxford, United Kingdom.
AD  - Faculty of Infectious and Tropical Diseases, London School of Hygiene and
      Tropical Medicine, London, United Kingdom.
FAU - Ng, Lee Ching
AU  - Ng LC
AD  - Environmental Health Institute, National Environment Agency, Singapore.
FAU - Phuoc, Nguyen Ngoc
AU  - Phuoc NN
AD  - Faculty of Fisheries, University of Agriculture and Forestry, Hue University, Hue
      City, Vietnam.
FAU - Sangwichian, Ornuma
AU  - Sangwichian O
AD  - Thailand Ministry of Public Health (MOPH)-US Centers for Disease Control and
      Prevention Collaboration (TUC), Nonthaburi, Thailand.
FAU - Sawatwong, Pongpun
AU  - Sawatwong P
AD  - Thailand Ministry of Public Health (MOPH)-US Centers for Disease Control and
      Prevention Collaboration (TUC), Nonthaburi, Thailand.
FAU - Surin, Uraiwan
AU  - Surin U
AD  - Nakhon Phanom General Hospital, Nakhon Phanom Provincial Health Office, Nakhon
      Phanom, Thailand.
FAU - Tan, Thean Yen
AU  - Tan TY
AD  - Department of Laboratory Medicine, Changi General Hospital, Singapore.
FAU - Tang, Wen Ying
AU  - Tang WY
AD  - Molecular Biology Laboratory, Tan Tock Seng Hospital, Singapore.
FAU - Thuy, Nguyen Vu
AU  - Thuy NV
AD  - National Hospital for Obstetrics & Gynaecology, Hanoi, Vietnam.
FAU - Turner, Paul
AU  - Turner P
AUID- ORCID: http://orcid.org/0000-0002-1013-7815
AD  - Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global
      Health, University of Oxford, United Kingdom.
AD  - Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap,
      Cambodia.
FAU - Vongsouvath, Manivanh
AU  - Vongsouvath M
AD  - Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital,
      Vientiane, Lao People's Democratic Republic.
FAU - Zhang, Defeng
AU  - Zhang D
AD  - Key Laboratory of Tropical & Subtropical Fishery Resource Application &
      Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries
      Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, People's
      Republic of China.
FAU - Whistler, Toni
AU  - Whistler T
AUID- ORCID: http://orcid.org/0000-0002-7250-908X
AD  - Thailand Ministry of Public Health (MOPH)-US Centers for Disease Control and
      Prevention Collaboration (TUC), Nonthaburi, Thailand.
AD  - Division of Global Health Protection, Centers for Disease Control and Prevention,
      Atlanta, Georgia, United States of America.
FAU - Chen, Swaine L
AU  - Chen SL
AUID- ORCID: http://orcid.org/0000-0002-0107-2861
AD  - Department of Medicine, Yong Loo Lin School of Medicine, National University of
      Singapore, Singapore.
AD  - Infectious Diseases Group, Genome Institute of Singapore, Singapore.
LA  - eng
PT  - Journal Article
DEP - 20190627
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
SB  - IM
PMC - PMC6597049
COIS- I have read the journal's policy and the authors of this manuscript have the
      following competing interests: SLC and TB are named applicants on a patent for
      the ST83-specific PCR test used in this study.
EDAT- 2019/06/28 06:00
MHDA- 2019/06/28 06:00
CRDT- 2019/06/28 06:00
PHST- 2019/01/09 00:00 [received]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2019/06/28 06:00 [entrez]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/06/28 06:00 [medline]
AID - 10.1371/journal.pntd.0007421 [doi]
AID - PNTD-D-19-00013 [pii]
PST - epublish
SO  - PLoS Negl Trop Dis. 2019 Jun 27;13(6):e0007421. doi:
      10.1371/journal.pntd.0007421. eCollection 2019 Jun.