PMID- 31138611
OWN - NLM
STAT- In-Data-Review
LR  - 20190807
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 87
IP  - 8
DP  - 2019 Aug
TI  - Bacterial Microcompartment-Mediated Ethanolamine Metabolism in Escherichia coli
      Urinary Tract Infection.
LID - e00211-19 [pii]
LID - 10.1128/IAI.00211-19 [doi]
AB  - Urinary tract infections (UTIs) are common and in general are caused by
      intestinal uropathogenic Escherichia coli (UPEC) ascending via the urethra.
      Microcompartment-mediated catabolism of ethanolamine, a host cell breakdown
      product, fuels the competitive overgrowth of intestinal E. coli, both pathogenic 
      enterohemorrhagic E. coli and commensal strains. During a UTI, urease-negative E.
      coli bacteria thrive, despite the comparative nutrient limitation in urine. The
      role of ethanolamine as a potential nutrient source during UTIs is understudied. 
      We evaluated the role of the metabolism of ethanolamine as a potential nitrogen
      and carbon source for UPEC in the urinary tract. We analyzed infected urine
      samples by culture, high-performance liquid chromatography, reverse
      transcription-quantitative PCR, and genomic sequencing. The ethanolamine
      concentration in urine was comparable to the concentration of the most abundant
      reported urinary amino acid, d-serine. Transcription of the eut operon was
      detected in the majority of urine samples containing E. coli screened. All
      sequenced UPEC strains had conserved eut operons, while metabolic genotypes
      previously associated with UTI (dsdCXA, metE) were mainly limited to phylogroup
      B2. In vitro ethanolamine was found to be utilized as a sole source of nitrogen
      by UPEC strains. The metabolism of ethanolamine in artificial urine medium (AUM) 
      induced metabolosome formation and provided a growth advantage at the
      physiological levels found in urine. Interestingly, eutE (which encodes
      acetaldehyde dehydrogenase) was required for UPEC strains to utilize ethanolamine
      to gain a growth advantage in AUM, suggesting that ethanolamine is also utilized 
      as a carbon source. These data suggest that urinary ethanolamine is a significant
      additional carbon and nitrogen source for infecting E. coli strains.
CI  - Copyright (c) 2019 Dadswell et al.
FAU - Dadswell, Katherine
AU  - Dadswell K
AD  - School of Microbiology, University College Cork, Cork, Ireland.
FAU - Creagh, Sinead
AU  - Creagh S
AD  - Department of Microbiology, Cork University Hospital, Cork, Ireland.
FAU - McCullagh, Edward
AU  - McCullagh E
AD  - Department of Microbiology, Cork University Hospital, Cork, Ireland.
FAU - Liang, Mingzhi
AU  - Liang M
AD  - School of Biosciences, University of Kent, Canterbury, United Kingdom.
FAU - Brown, Ian R
AU  - Brown IR
AD  - School of Biosciences, University of Kent, Canterbury, United Kingdom.
FAU - Warren, Martin J
AU  - Warren MJ
AD  - School of Biosciences, University of Kent, Canterbury, United Kingdom.
FAU - McNally, Alan
AU  - McNally A
AD  - Institute of Microbiology and Infection, University of Birmingham, Birmingham,
      United Kingdom.
FAU - MacSharry, John
AU  - MacSharry J
AD  - School of Microbiology, University College Cork, Cork, Ireland J.MacSharry@ucc.ie
      m.prentice@ucc.ie.
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Prentice, Michael B
AU  - Prentice MB
AUID- ORCID: https://orcid.org/0000-0003-1117-2388
AD  - School of Microbiology, University College Cork, Cork, Ireland J.MacSharry@ucc.ie
      m.prentice@ucc.ie.
AD  - Department of Microbiology, Cork University Hospital, Cork, Ireland.
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - Department of Pathology, University College Cork, Cork, Ireland.
LA  - eng
PT  - Journal Article
DEP - 20190723
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
SB  - IM
PMC - PMC6652756
OTO - NOTNLM
OT  - Escherichia coli
OT  - ethanolamine
OT  - metabolosome
OT  - microcompartment
OT  - urinary tract infection
EDAT- 2019/05/30 06:00
MHDA- 2019/05/30 06:00
CRDT- 2019/05/30 06:00
PHST- 2019/04/02 00:00 [received]
PHST- 2019/05/16 00:00 [accepted]
PHST- 2019/05/30 06:00 [pubmed]
PHST- 2019/05/30 06:00 [medline]
PHST- 2019/05/30 06:00 [entrez]
AID - IAI.00211-19 [pii]
AID - 10.1128/IAI.00211-19 [doi]
PST - epublish
SO  - Infect Immun. 2019 Jul 23;87(8). pii: IAI.00211-19. doi: 10.1128/IAI.00211-19.
      Print 2019 Aug.