PMID- 31095579
OWN - NLM
STAT- In-Data-Review
LR  - 20190610
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 5
DP  - 2019
TI  - Short chain fatty acids enriched fermentation metabolites of soluble dietary
      fibre from Musa paradisiaca drives HT29 colon cancer cells to apoptosis.
PG  - e0216604
LID - 10.1371/journal.pone.0216604 [doi]
AB  - In this study, the prebiotic potential of soluble dietary fibre extracted from
      plantain inflorescence (PIF) was investigated. PIF demonstrated prebiotic
      potential by enhancing the growth of the probiotics under study and thereby
      hindered colon cancer development. The soluble dietary fibre from Musa
      paradisiaca inflorescence (PIF) was fermented using Lactobacillus casei and
      Bifidobacterium bifidum. The fermentation supernatants (LS and BS) were enriched 
      with short chain fatty acids (SCFA) and were able to initiate apoptotic
      signalling in HT29 colon cancer cells leading to cell death. Both BS and LS
      exhibited cytotoxic effect; induced DNA damage and enhanced generation of
      reactive oxygen species in HT29 cells leading to apoptosis. The induction of
      apoptosis was facilitated by the reduction of membrane potential of mitochondria 
      and ATP synthesis; enhanced delivery of cytochrome c and interference with the
      expression of pro/antiapoptotic proteins. BS, which exhibited better activity,
      was further analysed for the identification of differentially regulated proteins 
      by performing two dimensional electrophoresis and MALDI-TOF mass spectrometry.
      Results emphasized on the fact that, the exposure to BSalteredthe HT29 proteins
      expression, particularly the upregulation of apoptosis- inducing factor-AIFM1
      leading to apoptosis of HT29 cells.
FAU - K B, Arun
AU  - K B A
AD  - Agro Processing and Technology Division, National Institute for Interdisciplinary
      Science and Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
FAU - Madhavan, Aravind
AU  - Madhavan A
AD  - Microbial Processing and Technology Division, CSIR-NIIST, Thiruvananthapuram,
      Kerala, India.
FAU - T R, Reshmitha
AU  - T R R
AD  - Agro Processing and Technology Division, National Institute for Interdisciplinary
      Science and Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
FAU - Thomas, Sithara
AU  - Thomas S
AD  - Agro Processing and Technology Division, National Institute for Interdisciplinary
      Science and Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
FAU - Nisha, P
AU  - Nisha P
AUID- ORCID: http://orcid.org/0000-0002-9292-2226
AD  - Agro Processing and Technology Division, National Institute for Interdisciplinary
      Science and Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
LA  - eng
PT  - Journal Article
DEP - 20190516
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
PMC - PMC6522120
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/05/17 06:00
MHDA- 2019/05/17 06:00
CRDT- 2019/05/17 06:00
PHST- 2019/01/04 00:00 [received]
PHST- 2019/04/24 00:00 [accepted]
PHST- 2019/05/17 06:00 [entrez]
PHST- 2019/05/17 06:00 [pubmed]
PHST- 2019/05/17 06:00 [medline]
AID - 10.1371/journal.pone.0216604 [doi]
AID - PONE-D-18-33806 [pii]
PST - epublish
SO  - PLoS One. 2019 May 16;14(5):e0216604. doi: 10.1371/journal.pone.0216604.
      eCollection 2019.