PMID- 31072353
OWN - NLM
STAT- In-Process
LR  - 20190515
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 19
IP  - 1
DP  - 2019 May 9
TI  - Differences in colonic crypt morphology of spontaneous and colitis-associated
      murine models via second harmonic generation imaging to quantify colon cancer
      development.
PG  - 428
LID - 10.1186/s12885-019-5639-8 [doi]
AB  - BACKGROUND: Colorectal cancer remains the second leading cause of cancer death in
      the United States, and increased risk in patients with ulcerative colitis (a
      subset of inflammatory bowel disease) has motivated studies into early markers of
      dysplasia. The development of clinically translatable multiphoton imaging systems
      has allowed for the potential of in vivo label-free imaging of epithelial crypt
      structures via autofluorescence and/or second harmonic generation (SHG). SHG has 
      been used to investigate collagen structures in various types of cancer, though
      the changes that colorectal epithelial collagen structures undergo during tumor
      development, specifically colitis-associated tumors, have not been fully
      investigated. METHODS: This study used two murine models, using A/J mice, one for
      spontaneous carcinoma and one for colitis-associated carcinoma, to investigate
      and quantify SHG image features that could potentially inform future study
      designs of endoscopic multiphoton imaging systems. The spontaneous tumor model
      comprised a series of six weekly injections of azoxymethane (AOM model). The
      colitis-associated tumor model comprised a single injection of AOM, followed by
      cycles of drinking water with dissolved dextran sodium sulfate salt (AOM-DSS
      model). SHG images of freshly resected murine colon were acquired with a
      multiphoton imaging system, and image features, such as crypt size, shape and
      distribution, were quantified using an automated algorithm. RESULTS: The
      comparison of quantified features of crypt morphology demonstrated the ability of
      our quantitative image feature algorithms to detect differences between
      spontaneous (AOM model) and colitis-associated (AOM-DSS model) murine colorectal 
      tissue specimens. There were statistically significant differences in the mean
      and standard deviation of nearest neighbor (distance between crypts) and
      circularity between the Control cohort, AOM and AOM-DSS cohorts. We also saw
      significance between AOM and AOM-DSS cohorts when calculating nearest neighbor in
      images acquired at fixed depths. CONCLUSION: The results provide insight into the
      ability of SHG imaging to yield relevant data about the crypt microstructure in
      colorectal epithelium, specifically the potential to distinguish between
      spontaneous and colitis-associated murine models using quantification of crypt
      shape and distribution, informing future design of translational multiphoton
      imaging systems and protocols.
FAU - Prieto, Sandra P
AU  - Prieto SP
AUID- ORCID: http://orcid.org/0000-0002-2261-3065
AD  - Biomedical Engineering Department, University of Arkansas, Fayetteville, AR,
      72701, USA.
FAU - Reed, Cassandra L
AU  - Reed CL
AD  - Biomedical Engineering Department, University of Arkansas, Fayetteville, AR,
      72701, USA.
FAU - James, Haley M
AU  - James HM
AD  - Biomedical Engineering Department, University of Arkansas, Fayetteville, AR,
      72701, USA.
FAU - Quinn, Kyle P
AU  - Quinn KP
AD  - Biomedical Engineering Department, University of Arkansas, Fayetteville, AR,
      72701, USA.
FAU - Muldoon, Timothy J
AU  - Muldoon TJ
AD  - Biomedical Engineering Department, University of Arkansas, Fayetteville, AR,
      72701, USA. tmuldoon@uark.edu.
LA  - eng
GR  - R15 CA202662/CA/NCI NIH HHS/United States
GR  - 1R15CA202662-01/Foundation for the National Institutes of Health
GR  - N/A/Arkansas Biosciences Institute
PT  - Journal Article
DEP - 20190509
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
PMC - PMC6507102
OTO - NOTNLM
OT  - Azoxymethane
OT  - Colorectal cancer
OT  - Crypt morphology
OT  - Dextran sulfate sodium salt
OT  - Image analysis
OT  - Inflammatory bowel disease
OT  - Multiphoton
OT  - Second harmonic generation
EDAT- 2019/05/11 06:00
MHDA- 2019/05/11 06:00
CRDT- 2019/05/11 06:00
PHST- 2018/09/14 00:00 [received]
PHST- 2019/04/24 00:00 [accepted]
PHST- 2019/05/11 06:00 [entrez]
PHST- 2019/05/11 06:00 [pubmed]
PHST- 2019/05/11 06:00 [medline]
AID - 10.1186/s12885-019-5639-8 [doi]
AID - 10.1186/s12885-019-5639-8 [pii]
PST - epublish
SO  - BMC Cancer. 2019 May 9;19(1):428. doi: 10.1186/s12885-019-5639-8.