PMID- 31016725
OWN - NLM
STAT- Publisher
LR  - 20190424
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
DP  - 2019 Apr 23
TI  - MicroRNA-203 reinforces stemness properties in melanoma and augments
      tumorigenesis in vivo.
LID - 10.1002/jcp.28619 [doi]
AB  - One of the challenges encountered in microRNA (miRNA) studies is to observe their
      dual role in different conditions and cells. This leads to a tougher prediction
      of their behavior as gene expression regulators. miR-203 has been identified to
      play a negative role in the progression of malignant melanoma; however, it has
      been reported, with dual effect, as both an oncomiR and tumor suppressor miRNA in
      some malignancies, such as breast cancer, meanwhile, the role of miR-203 in
      melanoma stem cells or even metastatic cells is unclear. In the present study,
      after observation of upregulation of miR-203 in melanoma patient's serum and also
      melanospheres as cancer stem cells model, we examined its overexpression on the
      stemness potential and migration ability of melanoma cells. Our data demonstrated
      that the increased miR-203 level was significantly associated with significant
      increase in the ability of proliferation, colony and spheres formation,
      migration, and tumorigenesis in A375 and NA8 cells. All of these changes were
      associated with enhancement of BRAF, several epithelial to mesenchymal transition
      factors, and stemness genes. In conclusion, our results clearly determined that
      miR-203 could be down-regulateddownregulated in melanoma tissues but be
      overexpressed in melanoma stem cells. It has an important role as oncomiR and
      promote repopulation, tumorigenicity, self-renewal, and migration. Therefore, we 
      suggested overexpression of miR-203 as biomarker for early detection of
      metastasis. However, more studies are needed to validate our data.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Sahranavardfard, Parisa
AU  - Sahranavardfard P
AUID- ORCID: http://orcid.org/0000-0003-0309-3049
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
FAU - Firouzi, Javad
AU  - Firouzi J
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
FAU - Azimi, Masoumeh
AU  - Azimi M
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
FAU - Khosravani, Pardis
AU  - Khosravani P
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
FAU - Heydari, Raheleh
AU  - Heydari R
AD  - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center,
      Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti
      University of Medical Sciences, Tehran, Iran.
AD  - Department of Andrology, Reproductive Biomedicine Research Center, Royan
      Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
FAU - Emami Razavi, Amirnader
AU  - Emami Razavi A
AD  - Iran National Tumor Bank, The cancer institute of Iran, Tehran University of
      Medical Sciences, Tehran, Iran.
FAU - Dorraj, Mahshad
AU  - Dorraj M
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
FAU - Keighobadi, Faezeh
AU  - Keighobadi F
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
FAU - Ebrahimi, Marzieh
AU  - Ebrahimi M
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center,
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
LA  - eng
GR  - 91000426/Royan Institute
GR  - 91000426/Royan Institute for Stem Cell Biology and Technology
PT  - Journal Article
DEP - 20190423
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
OTO - NOTNLM
OT  - melanoma stem cell
OT  - miR-203
OT  - microRNA
OT  - self-renewal
OT  - tumorigenesis
EDAT- 2019/04/25 06:00
MHDA- 2019/04/25 06:00
CRDT- 2019/04/25 06:00
PHST- 2019/01/01 00:00 [received]
PHST- 2019/02/25 00:00 [revised]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/04/25 06:00 [entrez]
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
AID - 10.1002/jcp.28619 [doi]
PST - aheadofprint
SO  - J Cell Physiol. 2019 Apr 23. doi: 10.1002/jcp.28619.