PMID- 30999159
OWN - NLM
STAT- In-Process
LR  - 20190527
IS  - 1872-8464 (Electronic)
IS  - 0165-5876 (Linking)
VI  - 122
DP  - 2019 Jul
TI  - Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane
      in children.
PG  - 111-116
LID - S0165-5876(19)30170-3 [pii]
LID - 10.1016/j.ijporl.2019.04.008 [doi]
AB  - AIMS: Immunohistochemical analysis of retraction pocket pars tensa of tympanic
      membrane in children. Identification of signs typical for cholesteatoma and
      support of retraction theory of cholesteatoma. STUDY DESIGN: a prospective study 
      analysing 31 surgically removed retraction pockets. DEPARTMENT: University
      Hospital, Children's Medical Centre Methods: Retraction pockets processed by a
      standard process for immunohistochemical analysis. The observed findings were
      specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet
      endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium),
      MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67
      (cellular marker of proliferation). RESULTS: All observed parameters except for
      MMP9 had a significantly higher incidence in retraction pocket stage III compared
      to stage II according to Charachon. CONCLUSION: We described immunohistochemical 
      signs of retraction pocket pars tensa of tympanic membrane in children resulting 
      in cholesteatoma. All the observed signs occur in the structure of matrix and
      perimatrix of cholesteatoma. A significantly higher incidence of all observed
      parameters except from MMP9 was proved in retraction pocket stage III, unlike in 
      stage II. This observation proves the fact that retraction pocket is a
      progressive disease and is a procholesteatoma stage.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Urik, Milan
AU  - Urik M
AD  - Department of Paediatric Otorhinolaryngology, University Hospital Brno and
      Faculty of Medicine, Masaryk University Brno, Brno, Czech Republic. Electronic
      address: docttor.urik@gmail.com.
FAU - Hurnik, Pavel
AU  - Hurnik P
AD  - Department of Pathology, University Hospital Ostrava and Faculty of Medicine,
      University of Ostrava, Ostrava, Czech Republic.
FAU - Ziak, Dusan
AU  - Ziak D
AD  - Department of Pathology, University Hospital Ostrava and Faculty of Medicine,
      University of Ostrava, Ostrava, Czech Republic.
FAU - Machac, Josef
AU  - Machac J
AD  - Department of Paediatric Otorhinolaryngology, University Hospital Brno and
      Faculty of Medicine, Masaryk University Brno, Brno, Czech Republic.
FAU - Slapak, Ivo
AU  - Slapak I
AD  - Department of Paediatric Otorhinolaryngology, University Hospital Brno and
      Faculty of Medicine, Masaryk University Brno, Brno, Czech Republic.
FAU - Motyka, Oldrich
AU  - Motyka O
AD  - Nanotechnology Centre, VSB-Technical University of Ostrava, Ostrava, Poruba,
      Czech Republic; ENET - Energy Units for Utilization of Non-Traditional Energy
      Sources, VSB-Technical University of Ostrava, Ostrava, Poruba, Czech Republic.
FAU - Jabandziev, Petr
AU  - Jabandziev P
AD  - Department of Pediatrics, University Hospital Brno and Faculty of Medicine,
      Masaryk University Brno, Brno, Czech Republic.
LA  - eng
PT  - Journal Article
DEP - 20190411
PL  - Ireland
TA  - Int J Pediatr Otorhinolaryngol
JT  - International journal of pediatric otorhinolaryngology
JID - 8003603
OTO - NOTNLM
OT  - Chidren
OT  - Cholesteatoma
OT  - Immunohistochemistry
OT  - Pars tensa
OT  - Retraction pocket
EDAT- 2019/04/19 06:00
MHDA- 2019/04/19 06:00
CRDT- 2019/04/19 06:00
PHST- 2019/01/08 00:00 [received]
PHST- 2019/04/09 00:00 [accepted]
PHST- 2019/04/19 06:00 [pubmed]
PHST- 2019/04/19 06:00 [medline]
PHST- 2019/04/19 06:00 [entrez]
AID - S0165-5876(19)30170-3 [pii]
AID - 10.1016/j.ijporl.2019.04.008 [doi]
PST - ppublish
SO  - Int J Pediatr Otorhinolaryngol. 2019 Jul;122:111-116. doi:
      10.1016/j.ijporl.2019.04.008. Epub 2019 Apr 11.