PMID- 30952726
OWN - NLM
STAT- MEDLINE
DCOM- 20190422
LR  - 20190502
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 39
IP  - 4
DP  - 2019 Apr
TI  - Anti-MUC1 Aptamer as Carrier Tool of the Potential Radiosensitizer 1,10
      Phenanthroline in MCF-7 Breast Cancer Cells.
PG  - 1859-1867
LID - 10.21873/anticanres.13293 [doi]
AB  - BACKGROUND: Proteins overexpressed in malignant tissues form important targets in
      the development of targeted therapeutics, and aptamers comprise an important
      affinity agent for therapy and drug delivery. In this study, aberrantly expressed
      mucin 1 glycoprotein was investigated as a therapeutic target in a breast cancer 
      model. MATERIALS AND METHODS: In order to determine the feasibility of using an
      aptamer against mucin 1 (aptA) as carrier of the cytotoxic compound
      1,10-phenanthroline to MCF-7 cells, as a potential radiosensitizer, was studied
      in experiments using circular dichroism and rhodamine labelling by fluorescent
      microscopy and flow cytometry. RESULTS: 1,10-Phenanthroline can be intercalated
      within aptA when complexed with Fe(II) ions, with dissociation constant (Kd) of
      30 muM. The complex was subsequently capable of binding to and being internalised
      in MCF-7 breast cancer cells. CONCLUSION: aptA can carry 1,10-phenanthroline to
      cancer cells specifically and this complex represents a potential target-directed
      anticancer therapy.
CI  - Copyright(c) 2019, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Alves, Lais N
AU  - Alves LN
AD  - Laboratory of Radiobiology, Division of Medical Physics, Institute of
      Radioprotection and Dosimetry, Brazilian Nuclear Energy Commission, Rio de
      Janeiro, Brazil.
AD  - Institute of Technology in Immunobiologics (Bio-Manguinhos), Oswaldo Cruz
      Foundation, Rio de Janeiro, Brazil.
FAU - Missailidis, Sotiris
AU  - Missailidis S
AD  - Institute of Technology in Immunobiologics (Bio-Manguinhos), Oswaldo Cruz
      Foundation, Rio de Janeiro, Brazil.
FAU - Lage, Claudia A S
AU  - Lage CAS
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de
      Janeiro, Rio de Janeiro, Brazil.
FAU - DE Almeida, Carlos Eduardo B
AU  - DE Almeida CEB
AD  - Laboratory of Radiobiology, Division of Medical Physics, Institute of
      Radioprotection and Dosimetry, Brazilian Nuclear Energy Commission, Rio de
      Janeiro, Brazil cbonacos@ird.gov.br.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Drug Carriers)
RN  - 0 (Ferrous Compounds)
RN  - 0 (MUC1 protein, human)
RN  - 0 (Mucin-1)
RN  - 0 (Phenanthrolines)
RN  - 0 (Radiation-Sensitizing Agents)
RN  - 39R4TAN1VT (ferrous sulfate)
RN  - W4X6ZO7939 (1,10-phenanthroline)
SB  - IM
MH  - Aptamers, Nucleotide/chemistry/genetics/*metabolism
MH  - Breast Neoplasms/genetics/*metabolism/pathology/radiotherapy
MH  - *Drug Carriers
MH  - *Endocytosis
MH  - Feasibility Studies
MH  - Female
MH  - Ferrous Compounds/chemistry
MH  - Humans
MH  - MCF-7 Cells
MH  - Mucin-1/genetics/*metabolism
MH  - Phenanthrolines/chemistry/*metabolism/pharmacology
MH  - Radiation-Sensitizing Agents/*metabolism/pharmacology
OTO - NOTNLM
OT  - *1,10-phenanthroline
OT  - *Aptamer
OT  - *MUC1
OT  - *breast cancer
OT  - *radiosensitizers
EDAT- 2019/04/07 06:00
MHDA- 2019/04/23 06:00
CRDT- 2019/04/07 06:00
PHST- 2019/01/07 00:00 [received]
PHST- 2019/01/23 00:00 [revised]
PHST- 2019/01/29 00:00 [accepted]
PHST- 2019/04/07 06:00 [entrez]
PHST- 2019/04/07 06:00 [pubmed]
PHST- 2019/04/23 06:00 [medline]
AID - 39/4/1859 [pii]
AID - 10.21873/anticanres.13293 [doi]
PST - ppublish
SO  - Anticancer Res. 2019 Apr;39(4):1859-1867. doi: 10.21873/anticanres.13293.