PMID- 30864684
OWN - NLM
STAT- MEDLINE
DCOM- 20190813
LR  - 20190813
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 54
IP  - 5
DP  - 2019 May
TI  - Extracellular vesicledelivered miR5055p, as a diagnostic biomarker of early lung 
      adenocarcinoma, inhibits cell apoptosis by targeting TP53AIP1.
PG  - 1821-1832
LID - 10.3892/ijo.2019.4738 [doi]
AB  - Lung adenocarcinoma (LA) is the most commonly occurring histological type of
      nonsmall cell lung cancer. Diagnosis and treatment of LA remain a major clinical 
      challenge. In the present study, to identify early LA biomarkers, extracellular
      vesicles (EVs) were separated from the plasma samples from 153 patients with LA
      and 75 healthy controls. microRNA (miRNA) expression profiling was performed at
      the screening stage (5 patients with LA vs. 5 controls), followed by verification
      at the validation stage (40 patients with LA vs. 20 controls) using reverse
      transcriptionquantitative polymerase chain reaction (RTqPCR). The four disordered
      miRNAs (miR5055p, miR4863p, miR4863p and miR3823p) identified in the plasma EVs
      were further evaluated at the testing stage (108 patients with LA vs. 50
      controls) by RTqPCR. It was revealed that miR5055p was upregulated, whereas
      miR3823p was downregulated, in the EVs from patients with LA. Furthermore,
      miR5055p was also upregulated in tumor tissues, compared with adjacent nontumor
      control tissues. Subsequently, the direct targets of miR5055p were predicted
      using bioinformatics analyses, and verified by luciferase assay and
      immunoblotting. The present study determined that miR5055p functions as an
      oncogene, promoting lung cancer cell proliferation and inhibiting cancer cell
      apoptosis via the targeting of tumor protein P53regulated apoptosisinducing
      protein 1 (TP53AIP1). Finally, it was confirmed that miR5055p in plasma EVs could
      be delivered to lung cancer cells, inhibiting cell apoptosis and promoting cell
      proliferation by targeting TP53AIP1. In conclusion, the present study indicated
      that miRNA5055p functions as an oncogene that may be used as a novel biomarker
      for the diagnosis and treatment of LA.
FAU - Fang, Hua
AU  - Fang H
AD  - Department of Oncology, Beijing ChaoYang Hospital, Capital Medical University,
      Beijing 100020, P.R. China.
FAU - Liu, Yutao
AU  - Liu Y
AD  - Department of Medical Oncology, National Cancer Center/National Clinical Research
      Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking
      Union Medical College, Beijing 100021, P.R. China.
FAU - He, Yaohong
AU  - He Y
AD  - Department of Respiratory Medicine, Fuxing Hospital, The Eighth Clinical Medical 
      College, Capital Medical University, Beijing 100038, P.R. China.
FAU - Jiang, Yang
AU  - Jiang Y
AD  - Department of Thoracic Surgery, Fuxing Hospital, The Eighth Clinical Medical
      College, Capital Medical University, Beijing 100038, P.R. China.
FAU - Wei, Yaping
AU  - Wei Y
AD  - Department of Oncology, Capital Medical University, Beijing 100038, P.R. China.
FAU - Liu, Han
AU  - Liu H
AD  - Department of Oncology, Capital Medical University, Beijing 100038, P.R. China.
FAU - Gong, Yueqing
AU  - Gong Y
AD  - Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of
      Education, College of Life Sciences, Beijing Normal University, Beijing 100875,
      P.R. China.
FAU - An, Guangyu
AU  - An G
AD  - Department of Oncology, Beijing ChaoYang Hospital, Capital Medical University,
      Beijing 100020, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190301
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN505 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (P53AIP1 protein, human)
MH  - A549 Cells
MH  - Adenocarcinoma of Lung/diagnosis/*genetics
MH  - Adult
MH  - Aged
MH  - Apoptosis
MH  - Apoptosis Regulatory Proteins/*genetics
MH  - Biomarkers, Tumor/*genetics
MH  - Case-Control Studies
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Early Detection of Cancer
MH  - Extracellular Vesicles/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lung Neoplasms/diagnosis/*genetics
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Up-Regulation
EDAT- 2019/03/14 06:00
MHDA- 2019/08/14 06:00
CRDT- 2019/03/14 06:00
PHST- 2018/08/30 00:00 [received]
PHST- 2019/01/25 00:00 [accepted]
PHST- 2019/03/14 06:00 [pubmed]
PHST- 2019/08/14 06:00 [medline]
PHST- 2019/03/14 06:00 [entrez]
AID - 10.3892/ijo.2019.4738 [doi]
PST - ppublish
SO  - Int J Oncol. 2019 May;54(5):1821-1832. doi: 10.3892/ijo.2019.4738. Epub 2019 Mar 
      1.