PMID- 30809737
OWN - NLM
STAT- Publisher
LR  - 20190227
IS  - 1434-9949 (Electronic)
IS  - 0770-3198 (Linking)
DP  - 2019 Feb 26
TI  - Elevated circulating T cell subsets and cytokines expression in patients with
      rheumatoid arthritis.
LID - 10.1007/s10067-019-04465-w [doi]
AB  - OBJECTIVE: This study aimed to assess the role of different subsets of
      circulating follicular helper T cells (Tfh), central memory (TCM), effector
      memory (TEM), Naive T, chemokines, and cytokines in the pathogenesis of
      rheumatoid arthritis (RA). METHODS: Blood samples from RA patients (n = 44) and
      healthy controls (n = 37) were analyzed. The frequencies of circulating Tfh, TCM,
      TEM, and Naive T cell subsets were enumerated, and the expression of
      co-stimulatory molecules, such as inducible co-stimulator (ICOS) and programmed
      death-1 (PD1), on these cells was evaluated by flow cytometry. The disease state 
      in RA patients was assessed using the DAS28. Concentrations of C-reactive protein
      (CRP), erythrocyte sedimentation rate (ESR), anti-cyclic citrullinated peptide
      (anti-CCP), and rheumatoid factor (RF) were measured. Cytokines and chemokines,
      such as IL-1beta, TNF-alpha, IL-4, IL-6, IL-9, IL-17A, MCP-1, IL-10, IL-12p70,
      and IL-21, were measured by a cytometric beads array assay. RESULTS: The
      percentages of circulating PD1(+)ICOS(+) Tfh, PD1(+)ICOS(+) TEM, and
      PD1(+)ICOS(+) TCM of PBMCs from RA patients were higher than those in healthy
      controls. Furthermore, expression of circulating PD1(+)ICOS(+) Tfh, PD1(+)ICOS(+)
      TEM, and PD1(+)ICOS(+) TCM showed a positive correlation with DAS28. In addition,
      increased levels of IL-1beta, IL-6, and MCP-1 were detected in the patients with 
      RA compared to healthy controls. CONCLUSIONS: Elevated circulating T cell subsets
      and cytokines expression profile were observed in RA patients. IL-6, MCP-1, and
      IL-1beta were significantly increased in RA, and PD1(+)ICOS(+) TEM, PD1(+)ICOS(+)
      TCM, and PD1(+)ICOS(+) Tfh cell subsets were positively correlated with disease
      activity DAS28. Therefore, PD1(+)ICOS(+) TEM, PD1(+)ICOS(+) TCM, and
      PD1(+)ICOS(+) Tfh cells might serve an important role in the progression of RA.
FAU - Zhou, Haiyan
AU  - Zhou H
AD  - Department of Clinical Research Centre, The Affiliated Hospital of Guizhou
      Medical University, 550004, Guiyang, 550004, Guizhou Province, People's Republic 
      of China. zhouhaiyan12388@126.com.
AD  - Guizhou Medical University, Guiyang, 550004, Guizhou Province, People's Republic 
      of China. zhouhaiyan12388@126.com.
AD  - Department of Immunology and Rheumatology, The Third Affiliated Hospital of
      Guizhou Medical University, Guiyang, 550004, Guizhou Province, People's Republic 
      of China. zhouhaiyan12388@126.com.
FAU - Hu, Bailong
AU  - Hu B
AD  - Department of Anesthesiology, The Affiliated Hospital of Guizhou Medical
      University, Guiyang, 550004, Guizhou Province, People's Republic of China.
FAU - Zhaopeng, Zheng
AU  - Zhaopeng Z
AD  - Guizhou Medical University, Guiyang, 550004, Guizhou Province, People's Republic 
      of China.
AD  - Department of Oncology, Guizhou Provincial People's Hospital, Guiyang, 550004,
      Guizhou Province, People's Republic of China.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Immunology and Rheumatology, The Affiliated Hospital of Guizhou
      Medical University, Guiyang, 550004, Guizhou Province, People's Republic of
      China.
FAU - Zhong, Qin
AU  - Zhong Q
AD  - Department of Clinical Research Centre, The Affiliated Hospital of Guizhou
      Medical University, 550004, Guiyang, 550004, Guizhou Province, People's Republic 
      of China.
FAU - Fan, Youyang
AU  - Fan Y
AD  - Department of Immunology and Rheumatology, The Affiliated Hospital of Guizhou
      Medical University, Guiyang, 550004, Guizhou Province, People's Republic of
      China.
FAU - Li, Long
AU  - Li L
AD  - Department of Immunology and Rheumatology, The Third Affiliated Hospital of
      Guizhou Medical University, Guiyang, 550004, Guizhou Province, People's Republic 
      of China. gzyxyll@medmail.com.
AD  - Department of Immunology and Rheumatology, The Affiliated Hospital of Guizhou
      Medical University, Guiyang, 550004, Guizhou Province, People's Republic of
      China. gzyxyll@medmail.com.
LA  - eng
GR  - 81460254/National Natural Science Foundation of China
GR  - qiankehejichu[2018]1137/the Science and Technology Fund of Guizhou Provincial
      Health Department
GR  - [2017]30-10/Fund of Guiyang Science and Technology department
GR  - gzwjkj2017-1-016/Population and Family Planning Commission of Shandong Province
      (CN)
GR  - qiannankeheshezi[2017]73/Fund of Qiannan Science and Technology department
PT  - Journal Article
DEP - 20190226
PL  - Germany
TA  - Clin Rheumatol
JT  - Clinical rheumatology
JID - 8211469
OTO - NOTNLM
OT  - Follicular help T cells
OT  - Memory T cells
OT  - Rheumatoid arthritis
EDAT- 2019/02/28 06:00
MHDA- 2019/02/28 06:00
CRDT- 2019/02/28 06:00
PHST- 2018/08/07 00:00 [received]
PHST- 2019/02/03 00:00 [accepted]
PHST- 2019/01/12 00:00 [revised]
PHST- 2019/02/28 06:00 [entrez]
PHST- 2019/02/28 06:00 [pubmed]
PHST- 2019/02/28 06:00 [medline]
AID - 10.1007/s10067-019-04465-w [doi]
AID - 10.1007/s10067-019-04465-w [pii]
PST - aheadofprint
SO  - Clin Rheumatol. 2019 Feb 26. pii: 10.1007/s10067-019-04465-w. doi:
      10.1007/s10067-019-04465-w.