PMID- 30707326
OWN - NLM
STAT- Publisher
LR  - 20190201
IS  - 1434-9949 (Electronic)
IS  - 0770-3198 (Linking)
DP  - 2019 Feb 1
TI  - MiR-5571-3p and miR-135b-5p, derived from analyses of microRNA profile
      sequencing, correlate with increased disease risk and activity of rheumatoid
      arthritis.
LID - 10.1007/s10067-018-04417-w [doi]
AB  - OBJECTIVES: This study aimed to investigate microRNA (miRNA) expression profiles 
      in synovium tissues of rheumatoid arthritis (RA) patients by RNA sequencing and
      to evaluate the values of dysregulated miRNAs in RA diagnosis and monitoring.
      METHODS: Thirty RA patients who underwent knee arthroscopy and 30 controls with
      knee trauma who underwent surgery were consecutively recruited, and synovium
      tissue samples of both groups were obtained during surgeries. In the exploration 
      part, miRNA and mRNA expression profiles of 3 RA samples and 3 control samples
      were detected using RNA sequencing then followed by bioinformatic analyses. In
      the validation part, 5 candidate miRNA levels were detected by quantitative
      polymerase chain reaction (qPCR) in 30 RA patients and 30 control patients.
      RESULTS: In the exploration part, 78 miRNAs and 1582 mRNAs were upregulated while
      40 miRNAs and 1295 mRNAs were downregulated in synovium tissue samples of RA
      patients compared with those of controls. Furthermore, enrichment analyses
      revealed that these dysregulated miRNAs and mRNAs were mainly implicated in
      immune activities and inflammatory diseases such as leukocyte migration,
      complement activation, and RA. In the validation part, qPCR assay revealed that
      miR-5571-3p and miR-135b-5p expressions were increased in RA patients compared
      with those in controls and disclosed good predictive values for RA risk with high
      area under the curves (AUCs). Besides, both miR-5571-3p and miR-135b-5p levels
      were positively correlated with disease activity and inflammation level of RA.
      CONCLUSIONS: Analyses of miRNA expression profiles by sequencing indicate that
      miR-5571-3p and miR-135b-5p correlate with increased RA risk and activity.
FAU - Liu, Cailong
AU  - Liu C
AD  - Department of Orthopaedic Sports Medicine, The First Affiliated Hospital of
      Wenzhou Medical University, Wenzhou, China.
FAU - Pan, Axiao
AU  - Pan A
AD  - Department of Rheumatology and Immunology, The First Affiliated Hospital of
      Wenzhou Medical University, South Baixiang, Ouhai District, Wenzhou, 325000,
      China.
FAU - Chen, Xiaowei
AU  - Chen X
AD  - Department of Rheumatology and Immunology, The First Affiliated Hospital of
      Wenzhou Medical University, South Baixiang, Ouhai District, Wenzhou, 325000,
      China.
FAU - Tu, Jianxin
AU  - Tu J
AD  - Department of Rheumatology and Immunology, The First Affiliated Hospital of
      Wenzhou Medical University, South Baixiang, Ouhai District, Wenzhou, 325000,
      China.
FAU - Xia, Xiaoru
AU  - Xia X
AD  - Department of Rheumatology and Immunology, The First Affiliated Hospital of
      Wenzhou Medical University, South Baixiang, Ouhai District, Wenzhou, 325000,
      China. xxr7799@163.com.
FAU - Sun, Li
AU  - Sun L
AUID- ORCID: http://orcid.org/0000-0002-7845-9706
AD  - Department of Rheumatology and Immunology, The First Affiliated Hospital of
      Wenzhou Medical University, South Baixiang, Ouhai District, Wenzhou, 325000,
      China. grassandsun@126.com.
LA  - eng
GR  - Y20170060 and Y20170056/Science and Technology Project of Wenzhou
GR  - ZX-01-C2016029/Science and Technology Action Plan for Major Disease Control -
      Trauma Repair Special Project, National Health and Family Planning Commission
      Medical Science and Technology Development Research Center
PT  - Journal Article
DEP - 20190201
PL  - Germany
TA  - Clin Rheumatol
JT  - Clinical rheumatology
JID - 8211469
OTO - NOTNLM
OT  - Expression profiles
OT  - MicroRNA
OT  - RNA sequencing
OT  - Rheumatoid arthritis
OT  - Synovium tissue
EDAT- 2019/02/02 06:00
MHDA- 2019/02/02 06:00
CRDT- 2019/02/02 06:00
PHST- 2018/09/07 00:00 [received]
PHST- 2018/12/26 00:00 [accepted]
PHST- 2018/11/22 00:00 [revised]
PHST- 2019/02/02 06:00 [entrez]
PHST- 2019/02/02 06:00 [pubmed]
PHST- 2019/02/02 06:00 [medline]
AID - 10.1007/s10067-018-04417-w [doi]
AID - 10.1007/s10067-018-04417-w [pii]
PST - aheadofprint
SO  - Clin Rheumatol. 2019 Feb 1. pii: 10.1007/s10067-018-04417-w. doi:
      10.1007/s10067-018-04417-w.