PMID- 30698824
OWN - NLM
STAT- Publisher
LR  - 20190218
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
DP  - 2019 Jan 30
TI  - A novel and non-invasive approach utilising nasal washings for the detection of
      nasopharyngeal carcinoma.
LID - 10.1002/ijc.32173 [doi]
AB  - Nasopharyngeal carcinoma (NPC) is an epithelial cancer of the nasopharynx which
      is highly associated with Epstein-Barr virus (EBV). Worldwide, most of the top 20
      countries with the highest incidence and mortality rates of NPC are low- and
      middle-income countries. Many studies had demonstrated that EBV could be detected
      in the tissue, serum and plasma of NPC patients. In this study, we explored the
      potential of assays based on non-invasive nasal washings (NW) as a diagnostic and
      prognostic tool for NPC. A total of 128 patients were evaluated for NW EBV DNA
      loads and a subset of these samples were also tested for 27 EBV and human miRNAs 
      shortlisted from literature. EBV DNA and seven miRNAs showed area under the
      receiver operating characteristic curve (AUC) values of more than 0.7, suggestive
      of their potential utility to detect NPC. Logistic regression analyses suggested 
      that combination of two NW assays that test for EBNA-1 and hsa-miR-21 had the
      best performance in detecting NPC. The trend of NW EBV DNA load matched with
      clinical outcome of 71.4% (10 out of 14) NPC patients being followed-up. In
      summary, the non-invasive NW testing panel may be particularly useful for NPC
      screening in remote areas where healthcare facilities and otolaryngologists are
      lacking, and may encourage frequent testing of individuals in the high risk
      groups who are reluctant to have their blood tested. However, further validation 
      in an independent cohort is required to strengthen the utility of this testing
      panel as a non-invasive detection tool for NPC.
CI  - (c) 2019 The Authors. International Journal of Cancer published by John Wiley &
      Sons Ltd on behalf of UICC.
FAU - Tan, Geok Wee
AU  - Tan GW
AUID- ORCID: https://orcid.org/0000-0003-3872-3485
AD  - Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research,
      Ministry of Health Malaysia, Kuala Lumpur, Malaysia.
AD  - Department of Pathology and Medical Biology, University of Groningen, University 
      Medical Center Groningen, Groningen, The Netherlands.
FAU - Sivanesan, Vijaya Mohan
AU  - Sivanesan VM
AUID- ORCID: https://orcid.org/0000-0002-3223-9265
AD  - Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research,
      Ministry of Health Malaysia, Kuala Lumpur, Malaysia.
AD  - Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala
      Lumpur, Malaysia.
FAU - Abdul Rahman, Farah Ida
AU  - Abdul Rahman FI
AD  - Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research,
      Ministry of Health Malaysia, Kuala Lumpur, Malaysia.
FAU - Hassan, Faridah
AU  - Hassan F
AD  - Department of Otorhinolaryngology, Selayang Hospital, Ministry of Health
      Malaysia, Batu Caves, Selangor, Malaysia.
FAU - Hasbullah, Harissa Husainy
AU  - Hasbullah HH
AD  - Department of Oncology and Radiotherapy, Kuala Lumpur Hospital, Ministry of
      Health Malaysia, Kuala Lumpur, Malaysia.
AD  - Department of Internal Medicine, Faculty of Medicine, UiTM Selangor, Shah Alam,
      Selangor, Malaysia.
FAU - Ng, Ching-Ching
AU  - Ng CC
AD  - Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala
      Lumpur, Malaysia.
FAU - Khoo, Alan Soo-Beng
AU  - Khoo AS
AUID- ORCID: https://orcid.org/0000-0002-8268-5271
AD  - Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research,
      Ministry of Health Malaysia, Kuala Lumpur, Malaysia.
FAU - Tan, Lu Ping
AU  - Tan LP
AUID- ORCID: https://orcid.org/0000-0002-7934-2462
AD  - Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research,
      Ministry of Health Malaysia, Kuala Lumpur, Malaysia.
AD  - Department of Medical Sciences, School of Healthcare and Medical Sciences, Sunway
      University, Subang Jaya, Selangor, Malaysia.
LA  - eng
GR  - NMRR-11-597-9667/Ministry of Health Malaysia
PT  - Journal Article
DEP - 20190130
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
OTO - NOTNLM
OT  - EBV DNA load
OT  - microRNAs
OT  - nasal washings
OT  - nasopharyngeal carcinoma
EDAT- 2019/01/31 06:00
MHDA- 2019/01/31 06:00
CRDT- 2019/01/31 06:00
PHST- 2018/09/18 00:00 [received]
PHST- 2019/01/01 00:00 [revised]
PHST- 2019/01/22 00:00 [accepted]
PHST- 2019/01/31 06:00 [pubmed]
PHST- 2019/01/31 06:00 [medline]
PHST- 2019/01/31 06:00 [entrez]
AID - 10.1002/ijc.32173 [doi]
PST - aheadofprint
SO  - Int J Cancer. 2019 Jan 30. doi: 10.1002/ijc.32173.