PMID- 30684164
OWN - NLM
STAT- In-Process
LR  - 20190329
IS  - 1435-4373 (Electronic)
IS  - 0934-9723 (Linking)
VI  - 38
IP  - 4
DP  - 2019 Apr
TI  - Clinical features and cause analysis of false positive results of Aspergillus
      galactomannan assay in pulmonary cryptococcosis patients.
PG  - 735-741
LID - 10.1007/s10096-019-03469-3 [doi]
AB  - There have been conflicting reports of false positive galactomannan assay results
      in patients with systemic cryptococcosis. We sought to determine the frequency of
      GM positivity in patients with pulmonary cryptococcosis and confirm the source of
      this cross-reactivity in vitro. We conducted a retrospective study to elucidate
      the rate of galactomannan (GM) false positivity and cause in a cohort of 29
      patients with pulmonary cryptococcal disease. The production of GM cross-reacting
      substances by clinical isolates and laboratory isolates of C. neoformans was
      tested in vitro. The mean serum GM index (Platelia Aspergillus) in patients with 
      pulmonary cryptococcosis was 1.06, with 16 (55.2%) of patients having values
      above the positive cutoff value of 0.5. GM index values significantly decreased
      after treatment of cryptococcosis. There was no significant correlation between
      galactomannan and cryptococcal glucuronoxylomannan antigen (Eiken Latex test)
      results. Culture supernatants from clinical isolates and wild-type C. neoformans 
      did not react in the GM assay; however, growth in the presence of 6% sodium
      chloride induced the production of cross-reacting GM antigens in culture
      supernatants from clinical isolates, wild type and a
      glucuronoxylomannan-deficient mutant of C. neoformans, but not in culture
      supernatants from a galactoxylomannan-deficient strain. Our results support the
      cross-reactivity of cryptococcal galactoxylomannan with the serum GM assay in
      vitro and in patients with pulmonary cryptococcal infection.
FAU - Takazono, Takahiro
AU  - Takazono T
AUID- ORCID: http://orcid.org/0000-0002-0696-5386
AD  - Department of Infectious Diseases, Graduate School of Biomedical Sciences,
      Nagasaki University, Nagasaki, Japan. takahiro-takazono@nagasaki-u.ac.jp.
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan. takahiro-takazono@nagasaki-u.ac.jp.
AD  - Departments of Medicine, Microbiology and Immunology, McGill University,
      Montreal, Quebec, Canada. takahiro-takazono@nagasaki-u.ac.jp.
AD  - Infectious Diseases and Immunity in Global Health Program, Research Institute of 
      the McGill University Health Centre, Montreal, Quebec, Canada.
      takahiro-takazono@nagasaki-u.ac.jp.
FAU - Saijo, Tomomi
AU  - Saijo T
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Ashizawa, Nobuyuki
AU  - Ashizawa N
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Oshima, Kazuhiro
AU  - Oshima K
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Nishimura, Keitaro
AU  - Nishimura K
AD  - Department of Infectious Diseases, Graduate School of Biomedical Sciences,
      Nagasaki University, Nagasaki, Japan.
FAU - Tashiro, Masato
AU  - Tashiro M
AD  - Department of Infectious Diseases, Graduate School of Biomedical Sciences,
      Nagasaki University, Nagasaki, Japan.
FAU - Yamamoto, Kazuko
AU  - Yamamoto K
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Imamura, Yoshifumi
AU  - Imamura Y
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Miyazaki, Taiga
AU  - Miyazaki T
AD  - Department of Infectious Diseases, Graduate School of Biomedical Sciences,
      Nagasaki University, Nagasaki, Japan.
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Yanagihara, Katsunori
AU  - Yanagihara K
AD  - Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
FAU - Mukae, Hiroshi
AU  - Mukae H
AD  - Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki,
      Japan.
FAU - Izumikawa, Koichi
AU  - Izumikawa K
AD  - Department of Infectious Diseases, Graduate School of Biomedical Sciences,
      Nagasaki University, Nagasaki, Japan.
FAU - Sheppard, Donald C
AU  - Sheppard DC
AD  - Departments of Medicine, Microbiology and Immunology, McGill University,
      Montreal, Quebec, Canada.
AD  - Infectious Diseases and Immunity in Global Health Program, Research Institute of 
      the McGill University Health Centre, Montreal, Quebec, Canada.
LA  - eng
GR  - FDN 159902/Foundation award from the Canadian Institutes of Health Research
PT  - Journal Article
DEP - 20190125
PL  - Germany
TA  - Eur J Clin Microbiol Infect Dis
JT  - European journal of clinical microbiology & infectious diseases : official
      publication of the European Society of Clinical Microbiology
JID - 8804297
OTO - NOTNLM
OT  - Aspergillus
OT  - Cryptococcosis
OT  - Cryptococcus neoformans
OT  - False positive
OT  - Galactomannan
OT  - Galactoxylomannan
EDAT- 2019/01/27 06:00
MHDA- 2019/01/27 06:00
CRDT- 2019/01/27 06:00
PHST- 2018/11/27 00:00 [received]
PHST- 2019/01/02 00:00 [accepted]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/01/27 06:00 [medline]
PHST- 2019/01/27 06:00 [entrez]
AID - 10.1007/s10096-019-03469-3 [doi]
AID - 10.1007/s10096-019-03469-3 [pii]
PST - ppublish
SO  - Eur J Clin Microbiol Infect Dis. 2019 Apr;38(4):735-741. doi:
      10.1007/s10096-019-03469-3. Epub 2019 Jan 25.