PMID- 30680573
OWN - NLM
STAT- MEDLINE
DCOM- 20190712
LR  - 20190712
IS  - 1435-4373 (Electronic)
IS  - 0934-9723 (Linking)
VI  - 38
IP  - 4
DP  - 2019 Apr
TI  - Implementation of multiplex PCR diagnostics for gastrointestinal pathogens linked
      to increase of notified Shiga toxin-producing Escherichia coli cases in Norway,
      2007-2017.
PG  - 801-809
LID - 10.1007/s10096-019-03475-5 [doi]
AB  - The aim of this study was to investigate implementation of multiplex PCR assays
      (broad screening PCR) on the distribution and characteristics of notified Shiga
      toxin-producing Escherichia coli (STEC) cases in Norway, 2007-2017. We described 
      STEC cases notified to the Norwegian Surveillance System for Communicable
      Diseases (MSIS), 2007-2017 and categorised cases as high-virulent, low-virulent
      or unclassifiable STEC infections based on guidelines for follow-up of STEC
      cases. We conducted descriptive analysis and time series analysis allowing for
      trends and seasonality, and calculated adjusted incidence rate ratios (aIRR)
      using negative binomial regression for laboratories with and without broad
      screening PCR. A total of 1458 STEC cases were notified to MSIS (2007-2017),
      median age 21 years, 51% female. Cases were categorised as having 475 (33%)
      high-virulent, 652 (45%) low-virulent, and 331 (23%) unclassifiable STEC
      infections. We observed a higher increasing monthly trend in cases (aIRR = 1.020;
      95% CI 1.016-1.024) notified from laboratories with broad screening PCR (n = 4)
      compared to laboratories (n = 17) without (aIRR = 1.011; 95% CI 1.007-1.014).
      Notification of low-virulent STEC infections increased from laboratories with
      broad screening PCR. The increase in notified STEC cases was prominent in cases
      categorised with a low-virulent STEC infection and largely attributable to
      unselective screening methods. We recommend NIPH to maintain differentiated
      control measures for STEC cases to avoid follow-up of low-virulent STEC
      infections. We recommend microbiological laboratories in Norway to consider a
      more cost-effective broad screening PCR strategy that enables differentiation of 
      high-virulent STEC infections.
FAU - Jenssen, Gaute Reier
AU  - Jenssen GR
AUID- ORCID: http://orcid.org/0000-0002-6973-4999
AD  - Department of Zoonotic, Food- and Waterborne Infections, Division for Infection
      Control and Environmental Health, Norwegian Institute of Public Health (NIPH),
      Postboks 4404, Nydalen, NO-0403, Oslo, Norway. g.r.jenssen@medisin.uio.no.
AD  - Faculty of Medicine, University of Oslo, Oslo, Norway.
      g.r.jenssen@medisin.uio.no.
AD  - Oslo University Hospital, Oslo, Norway. g.r.jenssen@medisin.uio.no.
FAU - Veneti, Lamprini
AU  - Veneti L
AD  - Department of Zoonotic, Food- and Waterborne Infections, Division for Infection
      Control and Environmental Health, Norwegian Institute of Public Health (NIPH),
      Postboks 4404, Nydalen, NO-0403, Oslo, Norway.
FAU - Lange, Heidi
AU  - Lange H
AD  - Department of Zoonotic, Food- and Waterborne Infections, Division for Infection
      Control and Environmental Health, Norwegian Institute of Public Health (NIPH),
      Postboks 4404, Nydalen, NO-0403, Oslo, Norway.
FAU - Vold, Line
AU  - Vold L
AD  - Department of Zoonotic, Food- and Waterborne Infections, Division for Infection
      Control and Environmental Health, Norwegian Institute of Public Health (NIPH),
      Postboks 4404, Nydalen, NO-0403, Oslo, Norway.
FAU - Naseer, Umaer
AU  - Naseer U
AD  - Department of Zoonotic, Food- and Waterborne Infections, Division for Infection
      Control and Environmental Health, Norwegian Institute of Public Health (NIPH),
      Postboks 4404, Nydalen, NO-0403, Oslo, Norway.
FAU - Brandal, Lin T
AU  - Brandal LT
AD  - Department of Zoonotic, Food- and Waterborne Infections, Division for Infection
      Control and Environmental Health, Norwegian Institute of Public Health (NIPH),
      Postboks 4404, Nydalen, NO-0403, Oslo, Norway.
LA  - eng
PT  - Journal Article
DEP - 20190124
PL  - Germany
TA  - Eur J Clin Microbiol Infect Dis
JT  - European journal of clinical microbiology & infectious diseases : official
      publication of the European Society of Clinical Microbiology
JID - 8804297
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Disease Outbreaks
MH  - Escherichia coli Infections/*diagnosis/epidemiology
MH  - Escherichia coli Proteins/genetics
MH  - Female
MH  - Humans
MH  - Incidence
MH  - Male
MH  - Middle Aged
MH  - *Multiplex Polymerase Chain Reaction
MH  - Norway/epidemiology
MH  - Regression Analysis
MH  - Seasons
MH  - Shiga-Toxigenic Escherichia coli/*isolation & purification
MH  - Virulence
MH  - Young Adult
PMC - PMC6424946
OTO - NOTNLM
OT  - High-virulent STEC
OT  - Incidence of STEC
OT  - Low-virulent STEC
OT  - Multiplex PCR panels
OT  - STEC diagnostic
EDAT- 2019/01/27 06:00
MHDA- 2019/07/13 06:00
CRDT- 2019/01/26 06:00
PHST- 2018/10/25 00:00 [received]
PHST- 2019/01/02 00:00 [accepted]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/07/13 06:00 [medline]
PHST- 2019/01/26 06:00 [entrez]
AID - 10.1007/s10096-019-03475-5 [doi]
AID - 10.1007/s10096-019-03475-5 [pii]
PST - ppublish
SO  - Eur J Clin Microbiol Infect Dis. 2019 Apr;38(4):801-809. doi:
      10.1007/s10096-019-03475-5. Epub 2019 Jan 24.