PMID- 30590281
OWN - NLM
STAT- MEDLINE
DCOM- 20190315
LR  - 20190315
IS  - 1872-8464 (Electronic)
IS  - 0165-5876 (Linking)
VI  - 118
DP  - 2019 Mar
TI  - Expression of collagens type I and V in healing rat's tympanic membrane.
PG  - 79-83
LID - S0165-5876(18)30631-1 [pii]
LID - 10.1016/j.ijporl.2018.12.020 [doi]
AB  - OBJECTIVES: Our preliminary study performed on perforated rat's tympanic membrane
      (TM) using Rat Wound Healing RT2 Profiler PCR Array showed significantly
      increased levels of mRNA for collagens type I and V. Enhanced expression of those
      genes does not assure that their protein products are indeed present, and in what
      quantity. Therefore, this study was undertaken to analyze the collagen type I and
      V content in the healing TM. METHODS: Sixty rats were used, of which 10 served as
      controls and the others had their TM perforated. The experimental animals were
      divided into five subgroups on the basis of time points (03, 06, 09, 14, 20 day
      after injury). Videootoscopy and histology were employed to assess the morphology
      of the healing process. The expression of collagen type I and V was evaluated
      using Western blot analysis. Tissue localization of collagens was determined by
      the immunofluorescence method. RESULTS: The collagen type I expression was three 
      times higher on the third day after injury and remained on that level for whole
      period of observation, up to day 20. The increase of the collagen type V
      expression was gradual, reaching the highest level on day 14 following injury. In
      comparison to the control TM statistically significant increase in the level of
      expression was observed starting from day 09 to the end of observation period. In
      healing TM immunofluorescent labeling of collagen type I and V was seen on the
      surface of remnants of previous lamina propria and in the loose proliferating
      fibrous tissue. On day 20 immunofluorescence was present mainly on the surface of
      thin connective tissue layers forming the scar in the place of previous
      perforation. CONCLUSION: Although the collagens type I and V are present only in 
      subepithelial layer in the normal rat's TM they play significant role in TM
      healing process.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Makuszewska, Maria
AU  - Makuszewska M
AD  - Department of Otolaryngology, Medical University of Warsaw, Banach 1a, 02-097,
      Warsaw, Poland.
FAU - Bonda, Tomasz
AU  - Bonda T
AD  - Department of General and Experimental Pathology, Medical University of
      Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland.
FAU - Cieslinska, Magdalena
AU  - Cieslinska M
AD  - Department of General and Experimental Pathology, Medical University of
      Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland.
FAU - Bialuk, Izabela
AU  - Bialuk I
AD  - Department of General and Experimental Pathology, Medical University of
      Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland.
FAU - Winnicka, Maria Malgorzata
AU  - Winnicka MM
AD  - Department of General and Experimental Pathology, Medical University of
      Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland.
FAU - Skotnicka, Bozena
AU  - Skotnicka B
AD  - Department of Pediatric Otolaryngology, Medical University of Bialystok,
      Waszyngtona 17, 15-274, Bialystok, Poland.
FAU - Hassmann-Poznanska, Elzbieta
AU  - Hassmann-Poznanska E
AD  - Department of Pediatric Otolaryngology, Medical University of Bialystok,
      Waszyngtona 17, 15-274, Bialystok, Poland. Electronic address:
      hassmann@umb.edu.pl.
LA  - eng
PT  - Journal Article
DEP - 20181218
PL  - Ireland
TA  - Int J Pediatr Otorhinolaryngol
JT  - International journal of pediatric otorhinolaryngology
JID - 8003603
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type V)
SB  - IM
MH  - Animals
MH  - Cicatrix/metabolism
MH  - Collagen Type I/*metabolism
MH  - Collagen Type V/*metabolism
MH  - Connective Tissue/metabolism
MH  - Male
MH  - Mucous Membrane/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Tympanic Membrane/injuries/*physiology
MH  - Tympanic Membrane Perforation/*metabolism/pathology
MH  - *Wound Healing
OTO - NOTNLM
OT  - Collagen type I
OT  - Collagen type V
OT  - Healing process
OT  - Perforation
OT  - Rats
OT  - Tympanic membrane
EDAT- 2018/12/28 06:00
MHDA- 2019/03/16 06:00
CRDT- 2018/12/28 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2018/12/14 00:00 [revised]
PHST- 2018/12/16 00:00 [accepted]
PHST- 2018/12/28 06:00 [pubmed]
PHST- 2019/03/16 06:00 [medline]
PHST- 2018/12/28 06:00 [entrez]
AID - S0165-5876(18)30631-1 [pii]
AID - 10.1016/j.ijporl.2018.12.020 [doi]
PST - ppublish
SO  - Int J Pediatr Otorhinolaryngol. 2019 Mar;118:79-83. doi:
      10.1016/j.ijporl.2018.12.020. Epub 2018 Dec 18.